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Bioptechs Micro Gas Humidifier 130708

Name: Bioptechs Micro Gas Humidifier 130708
Brand: Spectra Services
SKU: 130708
Price: 650 USD
Availability: InStock

The Bioptechs Gas Humidifier is an accessory to conveniently and inexpensively provide 100% humidification to a flow of air or mixed gas at a rate conducive to live-cell imaging.

Humidity control for Live-Cell imaging

Humidity control during live-cell, time-lapse experiments can be frustrating and lead to costly wasted time if you don’t have the right equipment and set up properly. It is important that the gas delivered to the chamber actually arrives at 100% humidity and the flow rate is appropriate for the size of the chamber. For single well time-lapse chambers, the airspace to culture medium ratio is low. Therefore, as a rule the volumetric gas exchange rate (assuming it is done properly) should be on the order of 1 to 5 volumes per hour to prevent pH or osmolarity shifts. The humidity in a closed cell chamber or dish reaches 100% within seconds of closure. Therefore, if you are applying a specialized gas mixture or 5% CO2 in air to the existing 100% RH airspace the inflow must be 100% RH or it will not take long to cause the pH and osmolarity of the media to change. The Bioptechs Micro-Gas Humidifier is an efficient, reliable, and convenient means to eliminate this obstacle for long-term imaging.

The Bioptechs Gas Humidifier warms water in a small containment vessel that the gas flows through creating a 100% RH atmosphere. The outflow of this gas is captured in the vessel, passed back through the heated base then close-coupled to the cell chamber thus preventing the RH from condensing before it reaches the cell chamber. If it is necessary to refill the water during an experiment, it has a Luer Slip refill cap so that water can be refilled with an ordinary syringe. It is safely powered by 12VDC and uses less than 2.5 watts. As a rule of thumb an unheated bubbler will only raise the RH around 10 to 15% above the inflow RH. However, a properly heated bubbler of the appropriate geometry will raise the inflow RH to 100%. It should also be appreciated that although you can produce 100% RH in a bottle or other device external to the cell containment structure it is necessary to prevent the air/gas flowing through the humidification device from condensing on its way to the cell chamber. Bioptechs addresses this problem by directly coupling the humidifier to the cell chamber thus providing a convenient, trouble free, and reliable solution. The Bioptechs Micro-Gas Humidifier along with a simple combination of a demand regulator and low flow peristaltic pump enables anyone to easily and cost effectively establish the proper gas and RH conditions for live cell microscopy.

How it works:

Humidifier System

This technique is ideal for long-term micro-observation of adherent cell culture, tissue slices in media, and suspended cells in CO2 dependent media on an inverted microscope or upright microscope when combined with the appropriate accessories.

Bioptechs Humidifier Components

Leur taper fill port cap, humidifier top with gas inlet port, humidifier glass, humidifier bottom with gas output port and power adapter port

All returns must be authorized by Spectra Services in order to insure proper credit and must be requested within 30 days of purchase. All returns are subject to a minimum 15% restocking charge. For returns not due to error on behalf of Spectra Services, the customer is responsible for all transportation fees related to the returned product. To ensure proper credit, each product return must include the following information:

Customer name and address
Purchase order number
Spectra Services shipping order number
Date of invoice
Item number of returned item(s)
Spectra Services return authorization number (RMA)
Reason for return

Non-Returnable Goods
Unfortunately, Spectra Services is unable to accept the following returns at this time:

Products not in completely resalable condition (including products with damaged, missing, or defaced labeling or packaging
Chemicals, reagents, diagnostics, sterile, or any controlled products (unless products do not meet specification
Laboratory apparatus or instruments that have been used or are without the original packaging, labeling and operating manuals
Refrigerated products or other perishables
Products which are not inventoried by Spectra Services and are not able to be returned to the manufacturer
Products purchased on a special order
Products not purchased from Spectra Services
Products with an expired shelf life or an expiration date too short for resale
Discontinued products

Spectra Services reserves the right to review this policy on a case-by-case basis.

Pre-Owned Products

Upon receipt of the pre-owned product(s), the customer has one week to evaluate the product to ensure it meets their expectations. If for some reason the customer decides to return the product, they may do so at any point during those seven (7) days for a full refund. No restocking fee will be assessed, however, the customer is responsible for the return shipping costs. Full credit will be issued upon receipt and inspection of the item(s) to ensure equipment was returned in the same condition it was initially shipped out in.

Important Information

All exchanges and returns require the completion of a Return Merchandise Authorization (RMA) form. Once completed, please return the merchandise immediately as the RMA number is only active for ten (10) days from the date of issuance.

Charges associated with returning items are the responsibility of the customer unless the item(s) arrived defective, damaged, or incorrect.

Spectra Services highly recommends that return packages be insured for full value and packaged securely to prevent shipping damage.

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