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PhaseView Alpha3 Light Sheet Microscope
    PhaseView Alpha3 Light Sheet Microscope
    Purchase PhaseView Alpha3 Light Sheet Microscope
    • PhaseView Alpha3 Light Sheet Microscope

      SKU: Alpha3
      Product Code: phaseview-alpha3
      Vendor: PhaseView
    • Quantity in Basket:  None

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    PhaseView ALPHA3


    Alpha3 is a new generation of light sheet microscope addressing the needs of high temporal resolution along with spatial high-resolution to achieve qualitative and quantitative 3D imaging of fixed or live biological specimen.

    From in-vivo imaging to large cleared samples, the Alpha3 microscope delivers unprecedented image quality while keeping the necessary flexibility and modularity expected for cutting-edge scientific research instruments.

    Typical applications of Alpha3 include in toto imaging of small animal models such as whole mouse embryos, morphogenesis and embryogenesis of model organisms: C.elegans, Drosophila, Zebra fish, live imaging of cell cultures, functional imaging of neuronal activity, fluorescence imaging of marine organisms or plant developmental biology.


    Features


    From in-vivo imaging to large cleared samples 
    Macro to micro view imaging, from whole organs at sub-cellular resolution to very small specimen; Compatible with all clearing solutions: aqueous buffers and organic solvents

    From very small to large specimen 
    Versatile chamber with easy access; Multiple accessories provided for sample mounting to accommodate a large variety of specimens

    Smart light sheet illuminators
    Multi-directional light sheet performing real time laser focus sweeping to extend the thinnest focus area over the entire field of view, artefacts-free imaging and ultra fast Z-stacking

    Modular light sheet microscope 
    Flexible imaging system with a large selection of detection objectives: 2X to 60X,  air, dipping lenses, clearing objectives with correction collar for RI matching; Fully flexible for easy adaptation to any experiments; Open microscopy system for multimodal optical microscopy: FLIM, FRET, FRAP, photo ablation.


    Software


    Light source

    Laser combiner with up to 4 laser lines: fibered lasers CW / Laser diodes or DPSS
    Wavelength selection from 405 nm to 785nm, output power from 25 to 250mW.

    Light sheet

    Dual smart illuminators with fibered connection to laser combiner.
    Multi-directional light sheet with real time focus sweeping
    Chromatic correction 400 ‐ 632nm
    Minimum light sheet thickness 2µm, range: 2µm - 12µm, width: 2mm - 15mm

    Chamber & Holders

    Chamber dimensions : width 21mm, length 70mm height 25mm; volume < 15ml
    Chamber highly resistant to various corrosive medium, clearing agents, sea water, 
    Sample size from µm to cm range 
    Multiple holders for sample mounting: molds, coverslips , glass supports 
    Optional: temperature and CO2 controls. 

    Volume scanning

    Z motorized stage: range 15mm, precision 0.1µm, acquisition speed 40 fps
    Optional: XY motorized stages for tiling: range 15mm, precision 0.1µm
    Optional: ultra fast 3D scanning module (75 images /second)

    Detection unit

    Fluorescence microscope stand comprising objective slider 2 positions , eyepieces, video port, motorized filter wheel, multi-notch filter

    Detection lenses

    Large selection of long working distance objectives : air ,dipping lenses, clearing objectives with correction collar RI 1.33 - 1.56 
    Magnification: 2X to 60X 

    Image Sensor

    sCMOS 2048 x 2048 pixels, format 13 x 13 mm, 6.5µm square pixels., USB3 / CameraLink Interface

    Software

    QtSPIM software for stacks, XY tiling and time lapse acquisition. Easy export of raw images and metadata to open source or commercial 3rd party software

    PC

    Desktop i7-7700K 4.2Ghz – 2 x 16Go RAM - GPU GeForce GTX1060-6Go - Hard Disk 4 x 4To RAID0 - Hard Disk SSD 500Go , QHD Screen 31.5”, Windows 10 / 64b Pro

     


    Software


    From sample mounting to image acquisition, Alpha3 performs seamless light sheet imaging. The QtSPIM software provides clear and intuitive interface to collect X,Y,Z,θ,T,λ images at maximum speed. QtSPIM controls all image acquisition parameters. Along with a workstation grade computer with an optimized configuration, the microscopy system performs ultra fast acquisition Raw image data with their metadata are saved in 16 bits TIFF format, compatible with open source or commercial software for further 3D display and analysis