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PhaseView Alpha3 Light Sheet Microscope

Thumbnail Filmstrip of PhaseView Alpha3 Light Sheet Microscope Images

    PhaseView Alpha3 Light Sheet Microscope

    Vendor: PhaseView
    Part Number: phaseview-alpha3

    Product Documents

    PhaseView ALPHA3

    Alpha3 is a new generation of light sheet microscope addressing the needs of high temporal resolution along with spatial high-resolution to achieve qualitative and quantitative 3D imaging of fixed or live biological specimen.

    From in-vivo imaging to large cleared samples, the Alpha3 microscope delivers unprecedented image quality while keeping the necessary flexibility and modularity expected for cutting-edge scientific research instruments.

    Typical applications of Alpha3 include in toto imaging of small animal models such as whole mouse embryos, morphogenesis and embryogenesis of model organisms: C.elegans, Drosophila, Zebra fish, live imaging of cell cultures, functional imaging of neuronal activity, fluorescence imaging of marine organisms or plant developmental biology.


    From in-vivo imaging to large cleared samples 
    Macro to micro view imaging, from whole organs at sub-cellular resolution to very small specimen; Compatible with all clearing solutions: aqueous buffers and organic solvents

    From very small to large specimen 
    Versatile chamber with easy access; Multiple accessories provided for sample mounting to accommodate a large variety of specimens

    Smart light sheet illuminators
    Multi-directional light sheet performing real time laser focus sweeping to extend the thinnest focus area over the entire field of view, artefacts-free imaging and ultra fast Z-stacking

    Modular light sheet microscope 
    Flexible imaging system with a large selection of detection objectives: 2X to 60X,  air, dipping lenses, clearing objectives with correction collar for RI matching; Fully flexible for easy adaptation to any experiments; Open microscopy system for multimodal optical microscopy: FLIM, FRET, FRAP, photo ablation.


    Light source

    Laser combiner with up to 4 laser lines: fibered lasers CW / Laser diodes or DPSS
    Wavelength selection from 405 nm to 785nm, output power from 25 to 250mW.

    Light sheet

    Dual smart illuminators with fibered connection to laser combiner.
    Multi-directional light sheet with real time focus sweeping
    Chromatic correction 400 ‐ 632nm
    Minimum light sheet thickness 2µm, range: 2µm - 12µm, width: 2mm - 15mm

    Chamber & Holders

    Chamber dimensions : width 21mm, length 70mm height 25mm; volume < 15ml
    Chamber highly resistant to various corrosive medium, clearing agents, sea water, 
    Sample size from µm to cm range 
    Multiple holders for sample mounting: molds, coverslips , glass supports 
    Optional: temperature and CO2 controls. 

    Volume scanning

    Z motorized stage: range 15mm, precision 0.1µm, acquisition speed 40 fps
    Optional: XY motorized stages for tiling: range 15mm, precision 0.1µm
    Optional: ultra fast 3D scanning module (75 images /second)

    Detection unit

    Fluorescence microscope stand comprising objective slider 2 positions , eyepieces, video port, motorized filter wheel, multi-notch filter

    Detection lenses

    Large selection of long working distance objectives : air ,dipping lenses, clearing objectives with correction collar RI 1.33 - 1.56 
    Magnification: 2X to 60X 

    Image Sensor

    sCMOS 2048 x 2048 pixels, format 13 x 13 mm, 6.5µm square pixels., USB3 / CameraLink Interface


    QtSPIM software for stacks, XY tiling and time lapse acquisition. Easy export of raw images and metadata to open source or commercial 3rd party software


    Desktop i7-7700K 4.2Ghz – 2 x 16Go RAM - GPU GeForce GTX1060-6Go - Hard Disk 4 x 4To RAID0 - Hard Disk SSD 500Go , QHD Screen 31.5”, Windows 10 / 64b Pro



    From sample mounting to image acquisition, Alpha3 performs seamless light sheet imaging. The QtSPIM software provides clear and intuitive interface to collect X,Y,Z,θ,T,λ images at maximum speed. QtSPIM controls all image acquisition parameters. Along with a workstation grade computer with an optimized configuration, the microscopy system performs ultra fast acquisition Raw image data with their metadata are saved in 16 bits TIFF format, compatible with open source or commercial software for further 3D display and analysis

    All returns must be authorized by Spectra Services in order to insure proper credit and must be requested within 30 days of purchase. All returns are subject to a minimum 15% restocking charge. For returns not due to error on behalf of Spectra Services, the customer is responsible for all transportation fees related to the returned product. To ensure proper credit, each product return must include the following information:

    • Customer name and address
    • Purchase order number
    • Spectra Services shipping order number
    • Date of invoice
    • Item number of returned item(s)
    • Spectra Services return authorization number (RMA)
    • Reason for return

    Non-Returnable Goods
    Unfortunately, Spectra Services is unable to accept the following returns at this time:

    • Products not in completely resalable condition (including products with damaged, missing, or defaced labeling or packaging
    • Chemicals, reagents, diagnostics, sterile, or any controlled products (unless products do not meet specification
    • Laboratory apparatus or instruments that have been used or are without the original packaging, labeling and operating manuals
    • Refrigerated products or other perishables
    • Products which are not inventoried by Spectra Services and are not able to be returned to the manufacturer
    • Products purchased on a special order
    • Products not purchased from Spectra Services
    • Products with an expired shelf life or an expiration date too short for resale
    • Discontinued products

    Spectra Services reserves the right to review this policy on a case-by-case basis.

    Pre-Owned Products

    Upon receipt of the pre-owned product(s), the customer has one week to evaluate the product to ensure it meets their expectations. If for some reason the customer decides to return the product, they may do so at any point during those seven (7) days for a full refund. No restocking fee will be assessed, however, the customer is responsible for the return shipping costs. Full credit will be issued upon receipt and inspection of the item(s) to ensure equipment was returned in the same condition it was initially shipped out in.

    Important Information

    All exchanges and returns require the completion of a Return Merchandise Authorization (RMA) form. Once completed, please return the merchandise immediately as the RMA number is only active for ten (10) days from the date of issuance.

    Charges associated with returning items are the responsibility of the customer unless the item(s) arrived defective, damaged, or incorrect.

    Spectra Services highly recommends that return packages be insured for full value and packaged securely to prevent shipping damage.

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    Spectra Services, Inc.
    249 David Parkway
    Ontario, NY 14519 US
    Phone: 800-955-7732
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