Nikon Immersion oil 50cc MXA20234 has been replaced with MXA22166
Nikon manufactures two types of Immersion Oil for microscopy these being Type N and Type F. These oils are tested using Nikon objectives and therefore the performance cannot be guaranteed against lenses manufactured by other companies. The refractive indices of other manufactured oils may differ which will impair the results gained from using these with Nikon objectives. It is also important to note that oils should not be mixed as this will impair the performance.
Type N is general purpose oil used for imaging applications requiring oil immersion, which can incorporate techniques such as brightfield, darkfield, DIC and phase contrast.It is available in three sizes of 8ml, 50ml and 480ml, all supplied with a pipette for dispensing. Type F is considered to be the superior grade specifically designed for the ever growing need to improve signal to noise ratios in fluorescence microscopy. This oil is also recommended for use with Nikon’s proprietary Perfect Focus System (PFS) for long term time lapse experiments, The improved quality is due to the type of raw materials used in the oil which relates to a subsequent reduction of auto fluorescence caused by minerals in the oil. There is one size available at 30ml, which is supplied with a plastic pipette for dispensing.