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Thumbnail Filmstrip of Hamamatsu ORCA-R2 C10600-10B Images
Hamamatsu ORCA-R2 C10600-10B
Hamamatsu ORCA-R2 C10600-10B
The ORCA-R2 is quite possibly the most widely used and well-respected cooled CCD used in laboratories today.
For years it has been and remains a workhorse camera for researchers across the world in a variety of applications ranging from calcium imaging to spinning disc microscopy.
Versatility and image quality define the core character of this camera. At the heart of the ORCA-R2 is Hamamatsu’s proprietary ERÐ150 CCD.
With improved quantum efficiency in red shifted wavelengths compared to ICXÐ285 based cameras (56% vs. 32% at the Cy5 peak emission of 670 nm) and read noise of only 6 electrons, this camera can accommodate samples with dynamic range of up to 6000 to 1.
Cooled by air (or by water if desired), dark current is minimized so as to be insignificant even after minutes of exposure.
Images from the ORCA-R2 contain not only quantitatively relevant data but also possess an appealing visual quality that is simply not achieved by other cooled CCD cameras.
An array of one million pixels with full frame acquisition speeds of 16 frames per second and Hamamatsu’s ability to enable extended red sensitivity, the ORCA-R2 can rapidly image fluorescence from GFP to mCherry and Cy5. As with all Hamamatsu cameras, it is supported by our bullet proof DCAMÐAPI drivers (used by virtually every scientific imaging software package). The ORCA-R2 is simple to use and interacts seamlessly with automated microscopes and accessories. A proven, reliable and multipurpose camera, the ORCA-R2 delivers exceptional images and data.
Features
High Sensitivity
High quantum efficiency of over 70 %
This camera provides QE of over 70 %. This camera has high sensitivity at visible to near infrared region.
Dual light mode
This camera has dual light mode (low light mode and high light mode) for various imaging situations. Low light mode provides high sensitivity for broad wavelength from visible to near infrared.
UV sensitivity
This camera has sensitivity in UV wavelength over 300 nm.
Fast readout / low noise
Dual scan mode
This camera has selectable normal and scan modes for optimal image acquisition.
Fast readout speed of 16.2 frames/s (full resolution)
This camera provides a fast readout speed of 16.2 frames/sec at full resolution.
Fastest readout speed of 115.1 frames/s (with binning, sub-array)
The fastest frame rate is 115.1 frames/s using fast scan mode with 8x8 binning and 8 sub-array.
Low readout noise (Optimized circuit design)
This camera has a dual optimized circuit design for normal and fast scan respectively, in order to minimize readout noise. Values as low as 6 electrons (rms) at normal scan and 10 electrons (rms) at fast scan.
High performance cooling
Dual cooling
Dark current is significantly reduced by cooling the CCD with a peltier element (Thermo-electric cooling device). Air or watercooling
is selectable for optimal image acquisition.
High cooling performance down to -40 ℃ (Water cooling)
This camera has high cooling performance down to -40 ℃ in water-cooled mode with +20 ℃ circulating water. This camera provides significantly low dark current of 0.0005 electrons/pixel/s.
Superior air cooling
This camera provides high cooling performance down to -35 ℃ even in air cooling mode. The maintenance-free hermetic vacuum sealed chamber, new peltier element and optimal heat radiation design contribute to this high cooling performance. Furthermore,
the cooling fan can be stopped temporarily to avoid minimal vibrations or electromagnetic noise during image acquisition.
Contrast enhancement
Analog contrast enhancement
Analog gain and analog offset features are implemented. By combining these two features, it enables to enhance analog signal to obtain higher contrast image before converting to digital signal.
High dynamic range
Standard dynamic range - 3000:1
Standard dynamic range is 3000:1 at full spatial resolution in normal scan mode. This is higher than most comparable cameras.
High dynamic range mode - 6000:1
This mode offers an increased full well capacity of 36 000 electrons when binning. This mode can only be used in normal scan mode.
Applications
- Fluorescence microscopy
- Live cells expressing GFP
- Red to NIR fluorescence application
- Ratio imaging
- Fluorescence in situ hybridization (FISH)
- IR-DIC and fluorescence imaging
- Cell, tissue, pathology
- Time lapse fluorescence imaging
- TIRF microscopy, real-time confocal microscopy
- Failure analysis
- Semiconductor inspection
- X-ray scintillator readout
Configuration
Specifications
| Type number | C10600-10B |
|---|---|
| Camera head type | Hermetic vacuum-sealed head |
| Imaging device | ER-150 progressive scan interline CCD |
| Effective no. of pixels | 1344 (H)_1024 (V) |
| Cell size | 6.45 μm (H)_6.45 μm (V) |
| Effective area | 8.67 mm (H)_6.60 mm (V) |
| Full well capacity | High dynamic range mode*ÊOFF: 18 000 electrons (typ.) High dynamic range mode*ÊON: 36 000 electrons (typ.) |
| Readout speed | Normal scan mode: 8.5 frames/s Fast scan mode: 16.2 frames/s |
| Readout noise | Normal scan mode: 6 electrons (rms) typ. Fast scan mode: 10 electrons (rms) typ. |
| Pixel clock rate | Normal scan mode: 14.00 MHz/pixel Fast scan mode: 28.00 MHz/pixel |
| Exposure time | 10 μs to 4200 s |
| Cooling method | Forced-air cooled/Water cooled |
| Cooling temperature | Forced-air cooled: -35 ℃ Water cooled: -40 ℃ (Water temperature: +20 ℃) |
| Dark current | 0.0005 electrons/pixel/s (at -40 ℃) |
| Dynamic range | 3000 : 1 typ. (at Normal scan) |
| Analog gain | Yes (10 times max.) |
| Analog offset feature | Yes |
| Sub-array | Yes |
| Binning | 2_2, 4_4, 8_8 |
| Camera control | IIDC 1394-Based Digital Camera Specification Ver.1.31 |
| External trigger mode | Edge trigger, Level trigger, Synchronous readout trigger, Start trigger |
| Trigger output | Integ output, Programmable timing output, Trigger ready output |
| Interface | IEEE1394b-2002 |
| A/D converter | 12 bit/16 bit |
| Lens mount | C-mount |
| Power supply | AC100 V to AC240 V, 50 Hz/60 Hz |
| Power consumption | Approx. 60 VA |
| Ambient operating temperature | 0 ℃ to +40 ℃ |
| Ambient storage temperature | -10 ℃ to +50 ℃ |
| Ambient operating humidity | 70 % max. (with no condensation) |
* High dynamic range mode is only available in normal scan mode with binning.
Fastest readout speed
【Normal scan】(Unit: frames/s)
| Binning | Sub-array (Effective pixel of vertical direction) | |||||||
| 1024 | 512 | 256 | 128 | 64 | 32 | 16 | 8 | |
| 1_1 | 8.5 | 15.6 | 26.7 | 41.4 | 57.2 | 70.6 | 80.0 | 85.7 |
| 2_2 | 15.6 | 26.7 | 41.4 | 57.2 | 70.6 | 80.0 | 85.7 | 88.8 |
| 4_4 | 26.7 | 41.4 | 57.2 | 70.6 | 80.0 | 85.7 | 88.8 | 90.5 |
| 8_8 | 40.6 | 56.3 | 69.8 | 79.2 | 85.0 | 88.2 | 89.9 | 90.8 |
【Fast scan】(Unit: frames/s)
| Binning | Sub-array (Effective pixel of vertical direction) | |||||||
| 1024 | 512 | 256 | 128 | 64 | 32 | 16 | 8 | |
| 1_1 | 16.2 | 28.4 | 45.7 | 65.6 | 83.9 | 97.5 | 106.2 | 111.1 |
| 2_2 | 28.4 | 45.7 | 65.6 | 83.9 | 97.5 | 106.2 | 111.1 | 113.7 |
| 4_4 | 45.7 | 65.6 | 83.9 | 97.5 | 106.2 | 111.1 | 113.7 | 115.0 |
| 8_8 | 64.3 | 82.7 | 96.5 | 105.3 | 110.3 | 113.0 | 114.4 | 115.1 |
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