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CoolLed pT-100 LED illumination system for transmitted light applications

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    CoolLed pT-100 LED illumination system for transmitted light applications

    SKU: pT-100

    Vendor: CoolLed
    Part Number: cooled-pt-100

    CoolLed pT-100 LED illumination system for transmitted light applications


    The pT-100 is a compact and simple-to-use LED illumination system for transmitted light applications, including brightfield, darkfield, DIC, Dodt gradient contrast and phase contrast.

    The system is available in four variants to bring the benefits of LEDs to modern laboratories. A broad white system provides a safer and convenient alternative to halogen by reducing harmful UV exposure to the eyes and is ideal for long hours spent at the microscope, while three narrow bandwidth options (525, 635 and 770 nm) optimise image quality through increased contrast. The pT-100-WHT and pT-100-770 nm are also available as variants with a 3 mm liquid light guide attachment, which is ideal for electrophysiology applications.

    Utilising the highly successful pE-100wht technology, we have further developed our LED solutions for Transmitted Light Applications with the pT-100 range.  Whether you’re looking for a broad white 100 W halogen replacement or want to optimise the quality of your images through the use of narrower spectrum wavelengths, the pT-100 can meet your needs.

    CoolLED LED illumination technology offers a host of benefits over halogen systems, with instant on/off and irradiance control in 1% steps from the ergonomically designed manual control pod which can be positioned close at hand. Remote control is also available via a TTL trigger.

    A true 100 W Halogen replacement: pT-100-WHT

    Laboratories familiar with halogen lamps will find that CoolLED’s broad white achieves results which make the move to LEDs simple and apparent.  Colour temperature remains constant as irradiance is increased which makes colour balancing unnecessary and improves standardisation between sessions. Samples will appear identical even though irradiance could be up to five times greater.

    In addition, the pT-100-WHT does not produce unwanted light in the UV which makes it safer and more comfortable, as risk of eye damage is significantly lower.

    Narrow bandwidth solutions eliminate background noise

    White light LED systems using a phosphored LED can be a source of background noise when used as a transmitted light source, which reduces image contrast. Narrow bandwidth illumination systems remove this background source to improve image quality and enhance analysis – even in thicker samples and applications requiring infrared light.

    • Higher contrast images: pT-100-525
      Narrow bandwidth at 525 nm is optimised for the spectral response of most scientific cameras and reduces background noise from a phosphored LED.
    • Revealing details in thick samples: pT-100-635
      Narrow bandwidth at 635 nm provides deeper penetration, which is excellent for revealing detail in thicker samples.
    • Infrared illumination of thicker samples: pT-100-770
      Narrow bandwidth at 770 nm provides deeper sample penetration techniques that require the use of infrared light.
    Liquid Light Guide for added flexibility

    The pT-100-WHT and pT-100-770 nm are also available with a 3 mm liquid light guide as an alternative to using a direct attachment adaptor, offering two main benefits:

    • Enhancing accuracy of sensitive electrophysiology experiments as remote positioning allows the Illumination Systems to be placed outside of a faraday cage, avoiding electrical interference.
    • Added compatibility with microscopes which only feature a liquid light guide input, the collimating optics being an integral part of the microscope.
    • T-100-WHT:

      • Comfortable operation and reduced risk of eye damage as no unwanted UV
      • Spectral performance matched to 100 W halogen, creating consistent images
      • No variation in colour across irradiance range, removing the need to colour balance
      • Replaces 100 W halogen lamp housing on most current and older microscopes – quick & easy exchange
      • LLG version enhances accuracy for electrophysiology applications


      • A phosphor-free illumination centred around 525 nm optimised for the spectral response of most scientific cameras
      • Improved image contrast compared to white light source


      • A phosphor-free solution for brightfield imaging with a peak of 635 nm providing deeper penetration in thicker samples
      • Reduced phototoxicity of light-sensitive samples


      • A specialised solution for IR contrast techniques such as DIC, Dodt contrast etc.
      • Centred around 770 nm provides the optimal output for deeper sample penetration techniques requiring infrared light
      • LLG version enhances accuracy of electrophysiology applications using thick tissue slices
      • he system comprises a pT-100 LED Light Source, control pod, and power supply.

        Power requirements
        100-240 V a.c. 50/60 Hz

        Power consumption
        1 W (standby)
        20 W (full irradiance)

        pT-100 Light Source (direct fit): 66 mm (diameter) x 128 mm(l) Weight 0.42 kg
        pT-100 Control Pod: 102 mm(w) x 110 mm(d) x 50 mm(h) – Weight 0.55 kg
        pT-100 Power supply: 55 mm(w) x 95 mm(d) x 40 mm(h) – Weight 0.19 kg

        Environment & Safety
        • Mercury-free & Laser-free
        • Energy Efficient
        • Long lifetime
        • No bulb replacements
        • Quiet operation
        • No special disposal regulations or issues
        • Reduced risk of eye damage.  Care should be taken when viewing samples using the microscope eyepiece while the transmitted light source is switched on. Users should select the lowest irradiance setting on the pod before using the eyepiece and increase irradiance as necessary.

        Control pod for operation and access to settings and usage history.

        Manual control for instant on/off and irradiance control in 1% steps from 0 – 100%

        Via single TTL for on/off control using a BNC connection on the control pod.  Triggering speed <150 µs

        Direct fit to a microscope

        Specify a Microscope Adaptor from CoolLED’s range of transmitted port microscope adaptors.


        Industry-leading 36 months. Read more here. 

        All data correct at time of publication


    All returns must be authorized by Spectra Services in order to insure proper credit and must be requested within 30 days of purchase. All returns are subject to a minimum 15% restocking charge. For returns not due to error on behalf of Spectra Services, the customer is responsible for all transportation fees related to the returned product. To ensure proper credit, each product return must include the following information:

    • Customer name and address
    • Purchase order number
    • Spectra Services shipping order number
    • Date of invoice
    • Item number of returned item(s)
    • Spectra Services return authorization number (RMA)
    • Reason for return

    Non-Returnable Goods
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    • Products not in completely resalable condition (including products with damaged, missing, or defaced labeling or packaging
    • Chemicals, reagents, diagnostics, sterile, or any controlled products (unless products do not meet specification
    • Laboratory apparatus or instruments that have been used or are without the original packaging, labeling and operating manuals
    • Refrigerated products or other perishables
    • Products which are not inventoried by Spectra Services and are not able to be returned to the manufacturer
    • Products purchased on a special order
    • Products not purchased from Spectra Services
    • Products with an expired shelf life or an expiration date too short for resale
    • Discontinued products

    Spectra Services reserves the right to review this policy on a case-by-case basis.

    Pre-Owned Products

    Upon receipt of the pre-owned product(s), the customer has one week to evaluate the product to ensure it meets their expectations. If for some reason the customer decides to return the product, they may do so at any point during those seven (7) days for a full refund. No restocking fee will be assessed, however, the customer is responsible for the return shipping costs. Full credit will be issued upon receipt and inspection of the item(s) to ensure equipment was returned in the same condition it was initially shipped out in.

    Important Information

    All exchanges and returns require the completion of a Return Merchandise Authorization (RMA) form. Once completed, please return the merchandise immediately as the RMA number is only active for ten (10) days from the date of issuance.

    Charges associated with returning items are the responsibility of the customer unless the item(s) arrived defective, damaged, or incorrect.

    Spectra Services highly recommends that return packages be insured for full value and packaged securely to prevent shipping damage.

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