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Thumbnail Filmstrip of Lamotte BioPaddles - Tryptic Soy Agar (TSA) & Rose Bengal Chloramphenicol Agar (RB) 5552 Images
Lamotte BioPaddles - Tryptic Soy Agar (TSA) & Rose Bengal Chloramphenicol Agar (RB) 5552
The BioPaddles® TSA/RB can be used for the cultivation of a wide variety of aerobic and anaerobic microorganisms (TSA). Selective enumeration and cultivation of yeasts, molds and Actinomycetes from food and other surfaces. (RB)
Features
Tryptic Soy Agar (TSA)
In 1955, Leavitt et al. discovered that Tryptic Soy Agar facilitated vigorous growth of aerobic and anaerobic microorganisms. This is an enriched media, suitable to support fastidious heterotrophs.
Rose Bengal Chloramphenicol Agar (RB)
Selective medium for the enumeration of fungi. This formula is prepared with a neutral pH and supplemented with chloramphenicol as the selective agent in fungal medium.
Application
Tryptic Soy Agar is recommended in multiple water & wastewater applications, and numerous standard methods for food testing. TSA is commonly used as a maintenance medium for culture collections and testing bacterial contaminants in cosmetics. Rose Bengal Chloramphenicol Agar is recommended in Standard Methods for the Examination of Water and Wastewater for the enumeration of yeasts and molds from foods and water.
Specifications
BioPaddles® - Tryptic Soy Agar (TSA) & Rose Bengal Chloramphenicol Agar (RB)
| Test Factor: | Microorganisms |
| Type of Agar(s) | Tryptic Soy (TSA)/Rose Bengal (RB) Agars |
| # of Tests: | 10 |
Culture Controls
10-100 inoculum (CFU)
| Culture | TSA Agar | RB Agar |
|---|---|---|
| Aspergillus niger | GROWTH | GROWTH |
| Bacilus subtilis | GROWTH | INHIBITED |
| Candida albicans | GROWTH | INHIBITED |
| Escherichia coli | GROWTH | INHIBITED |
| Pseudomonas aeruginosa | GROWTH | INHIBITED |
| Staphylococcus aureus | GROWTH | INHIBITED |
Storage/Expiration
Store tightly sealed BioPaddles® in a cool, dry location. Shield from direct sunlight. Store BioPaddles® at room temperature (65 to 77°F/18 to 25°C). Avoid sudden temperature changes. Temperature fluctuations may result in condensation settling at the bottom of the vial. This will not affect the culture properties, but could reduce the shelf-life or cause the agar to separate from the plastic paddle support. Do not refrigerate. Refrigeration may result in water condensation. Avoid freezing. If freezing occurs, thaw for 3-6 hours in the refrigerator (40°F; 4.4°C). Freezing can promote excess water loss and variation in media surface due to crystal formation. Refer to Best Before End date (See: BBE stamped on vial). Discard if paddle agar appears oxidized and darker than the expected color or if contaminants appear. The expiration date is based on medium in an intact container that is stored as directed.
Sampling
Liquids: Twist to remove paddle from vial. Fill vial to 40 mL fill line with the liquid to be sampled. The 40 mL volume can be used to calculate Total Viable Count (TVC) and/or Total Colony Count (TCC). Replace paddle. Allow a contact time of 15 seconds. Remove the paddle. Empty the vial. Replace the paddle in the vial.
Surfaces: Twist to remove paddle from vial. Allow the paddle surface (10 cm2) to come into physical contact with the test surface. Recovery rate is about 50%. To ensure an accurate recovery, gently sweep (or touch) the paddle to cover a 20 cm2 area. Replace paddle in vial.
Incubation
Incubate at 30 - 35°C for 24-48 hours. Growth will be present on the TSA agar, as it is enriched and non-selective. Growth may or may not be present on the Rose Bengal agar after this brief period as it is selective for yeasts and molds which tend to like cooler temperatures and grow more slowly in general. For this reason, re-incubate and check again after 2 to 4 days.
Colony Measuring
Each BioPaddles® paddle has molded media attachment points that are 4mm in length (point-to-point). This feature provides a useful guidepost to estimating nearby colony size.
Disposal
Twist to remove paddle from vial. Fill vial to 40 mL fill line with 1:9 dilution of household bleach (5.25% sodium hypochlorite). Replace paddle in vial. Allow 15 minute contact time. Remove paddle. Discard bleach solution. Replace paddle in vial and dispose. Alternatively, loosen cap and microwave for 30 seconds, autoclave, or incinerate.
All returns must be authorized by Spectra Services in order to insure proper credit and must be requested within 30 days of purchase. All returns are subject to a minimum 15% restocking charge. For returns not due to error on behalf of Spectra Services, the customer is responsible for all transportation fees related to the returned product. To ensure proper credit, each product return must include the following information:
- Customer name and address
- Purchase order number
- Spectra Services shipping order number
- Date of invoice
- Item number of returned item(s)
- Spectra Services return authorization number (RMA)
- Reason for return
Non-Returnable Goods
Unfortunately, Spectra Services is unable to accept the following returns at this time:
- Products not in completely resalable condition (including products with damaged, missing, or defaced labeling or packaging
- Chemicals, reagents, diagnostics, sterile, or any controlled products (unless products do not meet specification
- Laboratory apparatus or instruments that have been used or are without the original packaging, labeling and operating manuals
- Refrigerated products or other perishables
- Products which are not inventoried by Spectra Services and are not able to be returned to the manufacturer
- Products purchased on a special order
- Products not purchased from Spectra Services
- Products with an expired shelf life or an expiration date too short for resale
- Discontinued products
Spectra Services reserves the right to review this policy on a case-by-case basis.
Pre-Owned Products
Upon receipt of the pre-owned product(s), the customer has one week to evaluate the product to ensure it meets their expectations. If for some reason the customer decides to return the product, they may do so at any point during those seven (7) days for a full refund. No restocking fee will be assessed, however, the customer is responsible for the return shipping costs. Full credit will be issued upon receipt and inspection of the item(s) to ensure equipment was returned in the same condition it was initially shipped out in.
Important Information
All exchanges and returns require the completion of a Return Merchandise Authorization (RMA) form. Once completed, please return the merchandise immediately as the RMA number is only active for ten (10) days from the date of issuance.
Charges associated with returning items are the responsibility of the customer unless the item(s) arrived defective, damaged, or incorrect.
Spectra Services highly recommends that return packages be insured for full value and packaged securely to prevent shipping damage.
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