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Accuris High Fidelity Master Mix, 200 Rxns PR1001-HF-200
For applications requiring highly accurate amplification, Accuris offers a range of high fidelity PCR options. Our proofreading enzymes are modified for better sensitivity and higher activity, and this ensures more reproducible performance with a wide range of targets.
- Up to 100X higher fidelity than Taq polymerase.
- Crude DNA sample will not inhibit amplification.
- Ideal for cloning, mutagenesis, sequencing and microarrays.
- Options for blunt end or A-overhang products.
- Optimized buffers include PCR enhancers.
Use our convenient PCR Selector Tool to determine the correct Accuris PCR Reagent for your applications.
Accuris High Fidelty Polymerase (2 tube formulation)
- Leaves a blunt end for blunt end vector cloning
- 50x fidelity of native Taq
- Ideal for complex targets up to 10kb
For applications requiring highly accurate amplification, and full control of enzyme concentration, choose our High Fidelity DNA polymerase kit. Our polymerase enzyme has been genetically modified for better solubility and higher activity across a broad range of ionic conditions, and will amplify a wide range of targets, including those that are GC or AT rich as well as crude DNA samples.
A 3′-5′ proofreading exonuclease activity and an error rate of 4.55×10-7 makes Accuris High Fidelity DNA Polymerase the perfect partner for cloning applications. The included 5X buffer contains an optimized concentration of dNTPs and enhancers for compatibility with a variety of amplicons up to 10kb in length.
Accuris High Fidelity Master Mix
- Leaves a blunt end for blunt end vector cloning
- 100x fidelity of native Taq
- Ideal for shorter, less complex targets
A 2x formulation which provides extreme sensitivity in low copy number assays with 100x the fidelity of native Taq, Accuris High Fidelity Master Mix is perfect for shorter, less complex targets. The 2x master-mix contains proprietary enhancers and a proof-reading component for trouble-free PCR reaction assembly and performance. High Fidelity Master Mix delivers a unique balance of PCR sensitivity, high fidelity, versatility, and tolerance to inhibitors.
The pre-optimized Master Mix is supplied in a single tube, reducing the number of pipetting steps while improving throughput and reproducibility. The highly efficient buffer formulation provides the ideal conditions for high-performance PCR.
Accuris High Fidelity, Hot Start Master Mix
- Leaves an A-overhang for TA cloning
- 10x fidelity of native Taq
- Ideal for difficult, high GC content sequences up to 10kb
Accuris High Fidelity Hot Start Mix is a hot start 2x formulation which provides excellent sensitivity in low-copy number assays with 10x higher fidelity than Taq polymerase. The 2x master-mix contains proprietary enhancers, and an antibody mediated hot-start polymerase with proof-reading component for trouble-free PCR reaction assembly and performance.
The pre-optimized Hot Start Master Mix is supplied in a single tube, reducing the number of pipetting steps while improving throughput and reproducibility. The highly efficient buffer formulation and hot-start blend provide the ideal conditions for high-performance PCR and inactivity at room temperature thereby eliminating non-specific amplification.
All returns must be authorized by Spectra Services in order to insure proper credit and must be requested within 30 days of purchase. All returns are subject to a minimum 15% restocking charge. For returns not due to error on behalf of Spectra Services, the customer is responsible for all transportation fees related to the returned product. To ensure proper credit, each product return must include the following information:
- Customer name and address
- Purchase order number
- Spectra Services shipping order number
- Date of invoice
- Item number of returned item(s)
- Spectra Services return authorization number (RMA)
- Reason for return
Non-Returnable Goods
Unfortunately, Spectra Services is unable to accept the following returns at this time:
- Products not in completely resalable condition (including products with damaged, missing, or defaced labeling or packaging
- Chemicals, reagents, diagnostics, sterile, or any controlled products (unless products do not meet specification
- Laboratory apparatus or instruments that have been used or are without the original packaging, labeling and operating manuals
- Refrigerated products or other perishables
- Products which are not inventoried by Spectra Services and are not able to be returned to the manufacturer
- Products purchased on a special order
- Products not purchased from Spectra Services
- Products with an expired shelf life or an expiration date too short for resale
- Discontinued products
Spectra Services reserves the right to review this policy on a case-by-case basis.
Pre-Owned Products
Upon receipt of the pre-owned product(s), the customer has one week to evaluate the product to ensure it meets their expectations. If for some reason the customer decides to return the product, they may do so at any point during those seven (7) days for a full refund. No restocking fee will be assessed, however, the customer is responsible for the return shipping costs. Full credit will be issued upon receipt and inspection of the item(s) to ensure equipment was returned in the same condition it was initially shipped out in.
Important Information
All exchanges and returns require the completion of a Return Merchandise Authorization (RMA) form. Once completed, please return the merchandise immediately as the RMA number is only active for ten (10) days from the date of issuance.
Charges associated with returning items are the responsibility of the customer unless the item(s) arrived defective, damaged, or incorrect.
Spectra Services highly recommends that return packages be insured for full value and packaged securely to prevent shipping damage.
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Order Questions:
If you need help or have any other questions concerning your orders, please click on the "Contact us" form above or call: 800-955-7732.
249 David Parkway
Ontario, NY 14519 US
Phone: 800-955-7732
I'm having a hard time searching for a product.
That's no problem. We're more than happy to assist you in your search. Give us a call, or drop us a line and let us know what you're looking for as best you can. We're always adding partner brands, and it's difficult to keep up with the thousands of products that get added to our lineup. Our sales specialists know our lineup best, and are most willing to help you find the solution(s) you need!
Why can't I complete my order online?
You most likely have one or more items in your shopping cart that are not priced. If you find that you can't complete an order, contact us via phone or email for further assistance.
What types of payments do you accept?
That's an easy one. We're very flexible when it comes to payment. Not only do we accept most major credit cards (Visa, Master Card, Discover, American Express), we also offer you the flexibility of paying by check, money order, wire transfers, and purchase orders. Work with any one of our sales specialists to figure out which method is best for you!
Do you charge sales tax?
Currently, we are authorized to collect sales tax in California, Connecticut, Florida, Indiana, Massachusetts, New Jersey, New York, Ohio, Pennsylvania, Texas and Washington. If we ship to any of these states, we are obligated to charge sales tax unless you provide us with a tax exempt certificate. You can either fax a copy of the certificate to our New York office (585.265.4374) or email it to ([email protected]).
Do you offer special pricing for government and educational entities?
Absolutely! Contact any one of our sales specialists to work out the details!
I just placed an order. Can I modify/cancel it?
We pride ourselves on providing you with a responsive and efficient buying experience. We are able to address your concerns in an accurate and timely fashion, and can usually ship your order within 24 hours of order placement. If you contact us within that window, we'll do our absolute best to accommodate your needs and concerns prior to shipment.
Do you accept international orders?
Of course! While we require a bank wire prior to shipment of the product on international orders, we assure you that the process is just as smooth for domestic orders. Take a look at our International Shipping Policy and contact us if we can assist.
Will I be charged for shipping?
Shipping charges will be added to your invoice unless you provide us with your UPS, DHL, or FedEx account number. For a more detailed insight into our shipping process, see our shipping information page.
I received a damaged shipment. What can I do?
Accidental damage during shipment can sometimes happen. When you take delivery of your freight, please inspect thoroughly for any visible damage to the outside of the shipping container before signing for it. If there are multiple pieces to the shipment, please account for all them. Should you find that any of the products are damaged or missing after receipt, contact us within three days to address the matter.
I'd like to make a return. How do I do that?
Take a look at our Return Policy, and then contact us to continue with the process. Let us know if you have any questions.
Can you guarantee pricing and availability?
While we attempt to list and provide you the most up-to-date pricing, we reserve the right to make changes. Additionally, we cannot guarantee that we will always have a product in stock, but we do our best to accommodate your needs. If you have questions regarding pricing or availability, please do not hesitate to ask.
Can you explain where you come up with the 'List Price,' please?
Our "List Price" represents the full retail price as suggested by the supplier or manufacturer, the price estimated based on comparable items offered elsewhere in the marketplace, a former price the item was offered for by Spectra Services, or the price estimated in accordance with industry standards. Please note that we do our best to provide accurate list prices, and that it may vary by geographic location.
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