Nikon Immersion Oil

Immersion oil increases the resolving power of the microscope by replacing the air gap between the immersion objective lens and cover glass with a high refractive index medium and reducing light refraction.

Nikon manufactures four types of Immersion Oil for microscopy. These oils have been developed and tested specifically for use with Nikon objectives and therefore their performance cannot be guaranteed for lenses manufactured by other companies. Conversely, if non-Nikon oils are to be used with Nikon lenses, care must be taken to ensure that the refractive index and dispersion value of the oil match the properties of Nikon optics. Mis-match will result in aberrations and impair objective performance. It is also important to note that oils should not be mixed, as this will also impair performance.