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Thumbnail Filmstrip of Sutter BV-10-B Micropipette Beveler Basic System-No Microscope or Impedance Meter Included Images
Sutter BV-10-B Micropipette Beveler Basic System
Elegant and simple to use, the BV-10 offers precision beveling of micropipette tips between 0.1 and 100µm. The unique abrasive plate drive system is vibration free for greater control of the beveling process. Beveling can be accomplished very rapidly and produces consistent tip diameters using the techniques as described by Brown and Flaming,ÊScience, August 1974, Vol. 185.
Intracellular recording electrodes can benefit from beveling because of:
- A reduction in the tip diameter by creation of the sharp point on the electrode
- A lowered electrical resistance of the electrode due to the larger cross sectional area of the lumen.
This greatly facilitates penetrating and holding very small or difficult cells. Microinjection needles also benefit from beveling by promoting entry into cells with minimal damage while at the same time enhancing the flow of material through the needle.
The basic beveling system consists of a stationary pedestal, optically flat to a half wave (250nm), surface mounted on a heavy baseplate. This serves as a bearing for an abrasive coated glass grinding plate, which is also flat to half a wave. The flat abrasive plate is coupled to a low vibration, slow-speed motor by means of magnetic fields to provide a wobble-free flat grinding surface. The abrasive plates are fabricated with a proprietary process which insures a consistent abrasive coating.
A 2-axis micromanipulator holds the pipette to be beveled and permits controlled advancement onto the abrasive surface. The bevel angle and speed of advancement are adjustable. An LED lamp with a gooseneck enhances the beveling operation by providing sharp illumination of the abrasive plate and pipette.
The basic system is completed with two abrasive plates of your choice, a wick with holder (for wet beveling), pedestal oil, degreasing fluid, and manual.
Two options are available for monitoring the beveling process, an 80X stereo microscope and an electrode impedance meter. Depending on your research application, one or both of these options may be desirable. For all micropipette applications, the swing mounted microscope enhances your control of pipette advancement onto the abrasive plate and allows for viewing of the beveling operation (scope resolution is not sufficient for viewing the actual bevel except in the case of very large tips). For microelectrode applications, the impedance meter is used to monitor the tip resistance during the beveling operation. The meter is an analog design, offering three resistance ranges (0-10, 0-100, 0-500 MOhm). Measurements are made at 12 Hz to minimize capacitive contributions to the impedance measured and provide a near-true DC resistance value. A rapid roll-off is used to reduce 50/60 Hz interference, allowing operation in a laboratory environment without screening.
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FEATURES
- Vibration-free, magnetically coupled beveling surface
- Abrasive surface optically flat to a half wave (250nm)
- Finest abrasive surface commercially available
- Synchronous clock motor insures stable rotation rate
- 7lb steel baseplate adds additional dampening
- Integrated LED lamp
- Robust micromanipulator controls bevel angle and advancement
TECHNICAL SPECIFICATIONS
Beveling RangeÊ
0.1µm through 100µm finished electrodes depending on abrasive plate used
Grinding Surface Variation
less than 1.0µm
Grinding SpeedÊ
60 RPM
Beveling Angle Range
5-90 degrees - adjustable
Micromanipulator
course drive: 0.075in / dial revolution
fine drive: 0.0004in / dial revolution
Dimensions
19in x 9in x 8in | 48cm x 22cm x 20cm
Weight
45 lbs | 20 kg
Electrical
115/230volts | 50/60 Hertz power line
RoHS Certified
All returns must be authorized by Spectra Services in order to insure proper credit and must be requested within 30 days of purchase. All returns are subject to a minimum 15% restocking charge. For returns not due to error on behalf of Spectra Services, the customer is responsible for all transportation fees related to the returned product. To ensure proper credit, each product return must include the following information:
- Customer name and address
- Purchase order number
- Spectra Services shipping order number
- Date of invoice
- Item number of returned item(s)
- Spectra Services return authorization number (RMA)
- Reason for return
Non-Returnable Goods
Unfortunately, Spectra Services is unable to accept the following returns at this time:
- Products not in completely resalable condition (including products with damaged, missing, or defaced labeling or packaging
- Chemicals, reagents, diagnostics, sterile, or any controlled products (unless products do not meet specification
- Laboratory apparatus or instruments that have been used or are without the original packaging, labeling and operating manuals
- Refrigerated products or other perishables
- Products which are not inventoried by Spectra Services and are not able to be returned to the manufacturer
- Products purchased on a special order
- Products not purchased from Spectra Services
- Products with an expired shelf life or an expiration date too short for resale
- Discontinued products
Spectra Services reserves the right to review this policy on a case-by-case basis.
Pre-Owned Products
Upon receipt of the pre-owned product(s), the customer has one week to evaluate the product to ensure it meets their expectations. If for some reason the customer decides to return the product, they may do so at any point during those seven (7) days for a full refund. No restocking fee will be assessed, however, the customer is responsible for the return shipping costs. Full credit will be issued upon receipt and inspection of the item(s) to ensure equipment was returned in the same condition it was initially shipped out in.
Important Information
All exchanges and returns require the completion of a Return Merchandise Authorization (RMA) form. Once completed, please return the merchandise immediately as the RMA number is only active for ten (10) days from the date of issuance.
Charges associated with returning items are the responsibility of the customer unless the item(s) arrived defective, damaged, or incorrect.
Spectra Services highly recommends that return packages be insured for full value and packaged securely to prevent shipping damage.
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249 David Parkway
Ontario, NY 14519 US
Phone: 585-265-4320
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