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Thumbnail Filmstrip of Nest 5-Layer Cell Culture Flask, Vent Cap, Straight Neck, Tc, Sterile 1/Pk, 8/Cs 731002 Images
Nest 5-Layer Cell Culture Flask, Vent Cap, Straight Neck, Tc, Sterile 1/Pk, 8/Cs 731002
Cap Style： Vent Cap
- Made of high clarity, 100% virgin polystyrene.
- Sterilized by E-beam, SAL=10^-6.
- Non-Pyrogenic, DNase/Rnase free.
- Growth area: 870 cm²、520cm²
- Individually Packaged in sterile bag.
1、Mix cell suspension with medium: Prepare cell suspension of required concentration in a container. Recommended volume is about 30- 50mL per layer.
2、Add the mixed liquid into the Multi-layer Flask slowly with serological pipettes. To avoid foam and bubbles, allow liquid stream to flow along the slope of the Multi-layer Flask. (Save a little
liquid in pipette each time.)
3、Tips: A 10 mL pipette allows media to be dispensed at the bottom of the vessel. A 25 mL pipette allows media to be dispensed just past the NEST Logo.
4、Hold the Multi-layer Flask upright with the Logo facing you and tilt clockwise to a 45° angle on a flat work surface to partition the liquid into each layer.
5、While holding the Multi-layer Flask at a 45° angle, gently lay it flat onto the work surface with logo facing up.
6、After placing the Multi-layer Flask flat on a work surface, gently rock back and forth and side-to-side to distribute cells evenly onto culture surfaces.
7、Tips: Be careful to avoid foaming of medium, and not to spill liquid from each layer.
8、Repeat Step 3 to put the flask quickly and slightly into the incubator . Then, lay it flat as shown in Step 4. You may choose to either aspirate or pour the media from Multi-layer Flask.
9、Aspirating method: To aspirate or remove media, tilt Multi-layer Flask, with the NEST Logo facing you, counter-clock wise to a 45° angle while inverting the Multi-Flask toward you. Then, tilt Multi-layer Flask to the right, continuing to aspirate all residual media.
10、Pouring method: With Logo facing you, pour spent media from Multi-layer Flask
11、Tips: Aspirate media using a NEST 2 mL or 10mL aspirating pipette.
12、Wash with buffer for one time and add dissociating reagent (≥5mL per layer). Then, follow Steps 3-4 to distribute to dissociating reagent to each layer.
13、Neutralize with inactivating solution and mix following Steps 3-4. Gently swirl to dislodge cells completely.
14、Follow Step 7 “Aspirating Method” protocol and collect cell suspension using a NEST 10mL serological pipette.
15、Follow Step 8 “Pouring Method”. Pour the cell suspension into a NEST conical tube.
16、Rinse with additional wash buffer if needed.
17、Search "NEST Multi-layer Flask" video on NEST website or Youtube.
All returns must be authorized by Spectra Services in order to insure proper credit and must be requested within 30 days of purchase. All returns are subject to a minimum 15% restocking charge. For returns not due to error on behalf of Spectra Services, the customer is responsible for all transportation fees related to the returned product. To ensure proper credit, each product return must include the following information:
- Customer name and address
- Purchase order number
- Spectra Services shipping order number
- Date of invoice
- Item number of returned item(s)
- Spectra Services return authorization number (RMA)
- Reason for return
Non-Returnable Goods
Unfortunately, Spectra Services is unable to accept the following returns at this time:
- Products not in completely resalable condition (including products with damaged, missing, or defaced labeling or packaging
- Chemicals, reagents, diagnostics, sterile, or any controlled products (unless products do not meet specification
- Laboratory apparatus or instruments that have been used or are without the original packaging, labeling and operating manuals
- Refrigerated products or other perishables
- Products which are not inventoried by Spectra Services and are not able to be returned to the manufacturer
- Products purchased on a special order
- Products not purchased from Spectra Services
- Products with an expired shelf life or an expiration date too short for resale
- Discontinued products
Spectra Services reserves the right to review this policy on a case-by-case basis.
Pre-Owned Products
Upon receipt of the pre-owned product(s), the customer has one week to evaluate the product to ensure it meets their expectations. If for some reason the customer decides to return the product, they may do so at any point during those seven (7) days for a full refund. No restocking fee will be assessed, however, the customer is responsible for the return shipping costs. Full credit will be issued upon receipt and inspection of the item(s) to ensure equipment was returned in the same condition it was initially shipped out in.
Important Information
All exchanges and returns require the completion of a Return Merchandise Authorization (RMA) form. Once completed, please return the merchandise immediately as the RMA number is only active for ten (10) days from the date of issuance.
Charges associated with returning items are the responsibility of the customer unless the item(s) arrived defective, damaged, or incorrect.
Spectra Services highly recommends that return packages be insured for full value and packaged securely to prevent shipping damage.
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Order Questions:
If you need help or have any other questions concerning your orders, please click on the "Contact us" form above or call: 800-955-7732.
249 David Parkway
Ontario, NY 14519 US
Phone: 800-955-7732
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You most likely have one or more items in your shopping cart that are not priced. If you find that you can't complete an order, contact us via phone or email for further assistance.
What types of payments do you accept?
That's an easy one. We're very flexible when it comes to payment. Not only do we accept most major credit cards (Visa, Master Card, Discover, American Express), we also offer you the flexibility of paying by check, money order, wire transfers, and purchase orders. Work with any one of our sales specialists to figure out which method is best for you!
Do you charge sales tax?
Currently, we are authorized to collect sales tax in California, Connecticut, Florida, Indiana, Massachusetts, New Jersey, New York, Ohio, Pennsylvania, Texas and Washington. If we ship to any of these states, we are obligated to charge sales tax unless you provide us with a tax exempt certificate. You can either fax a copy of the certificate to our New York office (585.265.4374) or email it to ([email protected]).
Do you offer special pricing for government and educational entities?
Absolutely! Contact any one of our sales specialists to work out the details!
I just placed an order. Can I modify/cancel it?
We pride ourselves on providing you with a responsive and efficient buying experience. We are able to address your concerns in an accurate and timely fashion, and can usually ship your order within 24 hours of order placement. If you contact us within that window, we'll do our absolute best to accommodate your needs and concerns prior to shipment.
Do you accept international orders?
Of course! While we require a bank wire prior to shipment of the product on international orders, we assure you that the process is just as smooth for domestic orders. Take a look at our International Shipping Policy and contact us if we can assist.
Will I be charged for shipping?
Shipping charges will be added to your invoice unless you provide us with your UPS, DHL, or FedEx account number. For a more detailed insight into our shipping process, see our shipping information page.
I received a damaged shipment. What can I do?
Accidental damage during shipment can sometimes happen. When you take delivery of your freight, please inspect thoroughly for any visible damage to the outside of the shipping container before signing for it. If there are multiple pieces to the shipment, please account for all them. Should you find that any of the products are damaged or missing after receipt, contact us within three days to address the matter.
I'd like to make a return. How do I do that?
Take a look at our Return Policy, and then contact us to continue with the process. Let us know if you have any questions.
Can you guarantee pricing and availability?
While we attempt to list and provide you the most up-to-date pricing, we reserve the right to make changes. Additionally, we cannot guarantee that we will always have a product in stock, but we do our best to accommodate your needs. If you have questions regarding pricing or availability, please do not hesitate to ask.
Can you explain where you come up with the 'List Price,' please?
Our "List Price" represents the full retail price as suggested by the supplier or manufacturer, the price estimated based on comparable items offered elsewhere in the marketplace, a former price the item was offered for by Spectra Services, or the price estimated in accordance with industry standards. Please note that we do our best to provide accurate list prices, and that it may vary by geographic location.
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