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Molecular Depot Fructosyl Amine-Oxygen Oxidoreductase P2010030

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    Purchase Molecular Depot Fructosyl Amine-Oxygen Oxidoreductase P2010030

    Molecular Depot Fructosyl Amine-Oxygen Oxidoreductase P2010030

    SKU: p2010030


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    Vendor: molecular depot
    Shipping Weight: 1.00 pounds
    Part Number: molecular-depot-p2010030
    Our Price:
    $350.00 $695.00
    Sale Savings: $345.00

    Molecular Depot Fructosyl Amine-Oxygen Oxidoreductase P2010030

    Fructosyl Amine-Oxygen Oxidoreductase is a highly pure enzyme (specific activity > 3U/mg) that catalyzes the oxidative deglycation of glycated low molecular weight amino acids to produce amino acids, glucosone and hydrogen peroxide (H2O2). This product has been used as a key component in glycated hemoglobin HbA1c assays. It has also been used in a wide array of other biochemical applications. Custom bulk amounts and bulk pricing for this product are available upon request.

    Fructosyl Amine-Oxygen Oxidoreductase
    Catalog number: P2010030
    Lot number: Batch Dependent
    Expiration Date: Batch dependent
    Amount: 100 U
    Molecular Weight or Concentration: > 3 U/mg (~ 88 kDa). One unit will produce 1.0 μmole of hydrogen peroxide per minute at pH 8.0 at 37 °C
    Supplied as: Lyophilized Powder
    Applications: a component in glycated hemoglobin HbA1c assays
    Storage: −20°C
    Keywords: Fructosyl amine:oxygen oxidoreductase, fructosyl amino acid oxidase, amadoriase, EC 1.5.3, EC 1.5.3.X, fructosyl-a-l-amino acid:oxygen oxidoreductase, FAOD
    Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.

    References

    1: Ferri S, Kim S, Tsugawa W, Sode K. Review of fructosyl amino acid oxidase engineering research: a glimpse into the future of hemoglobin A1c biosensing. J Diabetes Sci Technol. 2009 May 1;3(3):585-92.

    2: Sakaue R, Hiruma M, Kajiyama N, Koyama Y. Cloning and expression of fructosyl-amino acid oxidase gene from Corynebacterium sp. 2-4-1 in Escherichia coli. Biosci Biotechnol Biochem. 2002 Jun;66(6):1256-61.

    3: Jeong HY, Song MH, Back JH, Han DM, Wu X, Monnier V, Jahng KY, Chae KS. The veA gene is necessary for the inducible expression by fructosyl amines of the Aspergillus nidulans faoA gene encoding fructosyl amino acid oxidase (amadoriase, EC 1.5.3). Arch Microbiol. 2002 Nov;178(5):344-50.

    4: Hirokawa K, Kajiyama N. Recombinant agrobacterium AgaE-like protein with fructosyl amino acid oxidase activity. Biosci Biotechnol Biochem. 2002 Nov;66(11):2323-9.

    5: Yoshida N, Takatsuka K, Katsuragi T, Tani Y. Occurrence of fructosyl-amino acid oxidase-reactive compounds in fungal cells. Biosci Biotechnol Biochem. 2005 Jan;69(1):258-60.

    6: Fujiwara M, Sumitani J, Koga S, Yoshioka I, Kouzuma T, Imamura S, Kawaguchi T, Arai M. Alteration of substrate specificity of fructosyl-amino acid oxidase from Fusarium oxysporum. Appl Microbiol Biotechnol. 2007 Mar;74(4):813-9.

    7: Yoshida N, Akazawa S, Katsuragi T, Tani Y. Characterization of two fructosyl- amino acid oxidase homologs of Schizosaccharomyces pombe. J Biosci Bioeng. 2004;97(4):278-80.

    8: Yoshida N, Sakai Y, Serata M, Tani Y, Kato N. Distribution and properties of fructosyl amino acid oxidase in fungi. Appl Environ Microbiol. 1995 Dec;61(12):4487-9.

    9: Sakaue R, Kajiyama N. Thermostabilization of bacterial fructosyl-amino acid oxidase by directed evolution. Appl Environ Microbiol. 2003 Jan;69(1):139-45. 10: Yoshida N, Akazawa S, Karino T, Ishida H, Hata Y, Katsuragi T, Tani Y, Takagi H. Functional analysis of genes encoding putative oxidoreductases in Aspergillus oryzae, which are similar to fungal fructosyl-amino acid oxidase. J Biosci Bioeng. 2007 Nov;104(5):424-7.

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