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Molecular Depot Colorimetric Chymotrypsin Substrate I B2013117
Colorimetric Chymotrypsin Substrate I is a high quality trifluoroacetate salt and colorimetric substrate for chymotrypsin. This product has been used as molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request.
Colorimetric Chymotrypsin Substrate I
Catalog number:B2013117
Lot number:Batch Dependent
Expiration Date:Batch dependent
Amount:1 mg
Molecular Weight or Concentration:499.5 g/mol
Supplied as:Lyophilized Powder
Applications:molecular tool for various biochemical applications
Storage:-20°C
Keywords:Suc-GGF-pNA, Suc-Gly-Gly-Phe-pNA, Suc-Gly-Gly-Phe-p-Nitroanilide
Grade:Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um.
References:
1: McCartney CE, MacLeod JA, Greer PA, Davies PL. An easy-to-use FRET protein substrate to detect calpain cleavage in vitro and in vivo Biochim Biophys Acta Mol Cell Res. 2018 Feb;1865(2):221-230.
2: Boehnke N, Maynard HD. Design of modular dual enzyme-responsive peptides Biopolymers. 2017 Sep;108(5):10.1002/bip.23035.
3: Rinderknecht H, Fleming RM. A new, highly sensitive and specific assay for chymotrypsin Clin Chim Acta. 1975 Mar 10;59(2):139-46.
4: Shibao PY, Anjolette FA, Lopes NP, Arantes EC. First serine protease inhibitor isolated from Rhinella schneideri poison J Venom Anim Toxins Incl Trop Dis. 2015 Aug 13;21:30.
5: Yakoby N, Raskin I. A simple method to determine trypsin and chymotrypsin inhibitory activity J Biochem Biophys Methods. 2004 Jun 30;59(3):241-51.
6: Gupta R, Chen J, Srivastava OP. A serine-type protease activity of human lens βA3-crystallin is responsible for its autodegradation Mol Vis. 2010 Nov 2;16:2242-52.
7: Evensen NA, Braun PC. The effects of tea polyphenols on Candida albicans: inhibition of biofilm formation and proteasome inactivation Can J Microbiol. 2009 Sep;55(9):1033-9.
8: Huang C, Wei P, Fan K, Liu Y, Lai L. 3C-like proteinase from SARS coronavirus catalyzes substrate hydrolysis by a general base mechanism Biochemistry. 2004 Apr 20;43(15):4568-74.
9: Niinobe M, Hitomi Y, Fujii S. A sensitive colorimetric assay for various proteases using naphthyl ester derivatives as substrates J Biochem. 1980 Mar;87(3):779-83.
10: Lopes AR, Sato PM, Terra WR. Insect chymotrypsins: chloromethyl ketone inactivation and substrate specificity relative to possible coevolutional adaptation of insects and plants Arch Insect Biochem Physiol. 2009 Mar;70(3):188-203.
All returns must be authorized by Spectra Services in order to insure proper credit and must be requested within 30 days of purchase. All returns are subject to a minimum 15% restocking charge. For returns not due to error on behalf of Spectra Services, the customer is responsible for all transportation fees related to the returned product. To ensure proper credit, each product return must include the following information:
- Customer name and address
- Purchase order number
- Spectra Services shipping order number
- Date of invoice
- Item number of returned item(s)
- Spectra Services return authorization number (RMA)
- Reason for return
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Unfortunately, Spectra Services is unable to accept the following returns at this time:
- Products not in completely resalable condition (including products with damaged, missing, or defaced labeling or packaging
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- Laboratory apparatus or instruments that have been used or are without the original packaging, labeling and operating manuals
- Refrigerated products or other perishables
- Products which are not inventoried by Spectra Services and are not able to be returned to the manufacturer
- Products purchased on a special order
- Products not purchased from Spectra Services
- Products with an expired shelf life or an expiration date too short for resale
- Discontinued products
Spectra Services reserves the right to review this policy on a case-by-case basis.
Pre-Owned Products
Upon receipt of the pre-owned product(s), the customer has one week to evaluate the product to ensure it meets their expectations. If for some reason the customer decides to return the product, they may do so at any point during those seven (7) days for a full refund. No restocking fee will be assessed, however, the customer is responsible for the return shipping costs. Full credit will be issued upon receipt and inspection of the item(s) to ensure equipment was returned in the same condition it was initially shipped out in.
Important Information
All exchanges and returns require the completion of a Return Merchandise Authorization (RMA) form. Once completed, please return the merchandise immediately as the RMA number is only active for ten (10) days from the date of issuance.
Charges associated with returning items are the responsibility of the customer unless the item(s) arrived defective, damaged, or incorrect.
Spectra Services highly recommends that return packages be insured for full value and packaged securely to prevent shipping damage.
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