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Molecular Depot HEPES Ultra Pure B2010032U

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    Purchase Molecular Depot HEPES Ultra Pure B2010032U

    Molecular Depot HEPES Ultra Pure B2010032U

    SKU: b2010032u


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    Vendor: molecular depot
    Shipping Weight: 2.60 pounds
    Part Number: molecular-depot-b2010032u
    Our Price:
    $595.00 $995.00
    Sale Savings: $400.00

    Molecular Depot HEPES Ultra Pure B2010032U

    HEPES Ultra Pure, with extremely low levels (≤ 5 ppm) of iron, copper, zinc, lead, nickel, chromium. cobalt and cadmium. Multi-purpose buffer for most biotechnology applications. Buffering range between pH 6.8 to 8.2. Useful for buffer formulation, reagent formulation, tissue culture and more. Custom bulk orders are available upon request.

    Specifications

    Catalog number: B2010032U
    Lot number: Batch Dependent
    Expiration date: Batch Dependent
    Volume/Weight 50 g
    Molecular Formula C8H18N2O4S
    Molecular Weight 238.3
    CAS Number 7365-45-9
    Application: buffer formulation, reagent formulation, reagent stabilization
    Shelf-Life 2 years from date of manufacture
    Storage: Room temperature
    Keywords: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
    Grade Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um.
    References 1: Morgia F, Torti M, Montigiani M, Piscitelli C, Giallonardo A, Schimberni M,
    Giannini P, Sbracia M. Use of a medium buffered with N-hydroxyethylpiperazine-N-
    ethanesulfonate (HEPES) in intracytoplasmic sperm injection procedures is
    detrimental to the outcome of in vitro fertilization. Fertil Steril. 2006
    May;85(5):1415-9.
    2: Simpson JA, Cheeseman KH, Smith SE, Dean RT. Free-radical generation by
    copper ions and hydrogen peroxide. Stimulation by Hepes buffer. Biochem J. 1988
    Sep 1;254(2):519-23.3: Kasraian K, Kuzniar A, Earley D, Kamicker BJ, Wilson G, Manion T, Hong J,
    Reiber C, Canning P. Sustained in vivo activity of recombinant bovine
    granulocyte colony stimulating factor (rbG-CSF) using HEPES buffer. Pharm Dev
    Technol. 2001 Aug;6(3):441-7.4: Habib A, Tabata M. Oxidative DNA damage induced by HEPES
    (2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid) buffer in the presence
    of Au(III). J Inorg Biochem. 2004 Nov;98(11):1696-702.

    5: Vasconcelos MT, Azenha MA, Lage OM. Electrochemical evidence of surfactant
    activity of the hepes pH buffer which may have implications on trace metal
    availability to cultures in vitro. Anal Biochem. 1996 Oct 15;241(2):248-53.

    6: Lomonosova EE, Kirsch M, Rauen U, de Groot H. The critical role of Hepes in
    SIN-1 cytotoxicity, peroxynitrite versus hydrogen peroxide. Free Radic Biol Med.
    1998 Mar 1;24(4):522-8.

    7: Kirsch M, Lomonosova EE, Korth HG, Sustmann R, de Groot H. Hydrogen peroxide
    formation by reaction of peroxynitrite with HEPES and related tertiary amines.
    Implications for a general mechanism. J Biol Chem. 1998 May 22;273(21):12716-24.

    8: Cowan AI, Martin RL. Simultaneous measurement of pH and membrane potential in
    rat dorsal vagal motoneurons during normoxia and hypoxia: a comparison in
    bicarbonate and HEPES buffers. J Neurophysiol. 1995 Dec;74(6):2713-21.

    9: Svajger U, Jeras M. Optimal dendritic cell differentiation in rpmi media
    requires the absence of HEPES buffer. vv Immunol Invest.2011;40(4):413-26

    All returns must be authorized by Spectra Services in order to insure proper credit and must be requested within 30 days of purchase. All returns are subject to a minimum 15% restocking charge. For returns not due to error on behalf of Spectra Services, the customer is responsible for all transportation fees related to the returned product. To ensure proper credit, each product return must include the following information:

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