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Molecular Depot TAE Buffer 10X (Tris-acetate-EDTA) (Biotechnology Grade) B2010011
TAE Buffer (10X concentrate) used for agarose DNA electrophoresis. A 1X solution is obtained by diluting 1 volume of the provided TAE 10X concentrated TAE to 9 volumes of deionized water.
Specifications
| Catalog number: | B2010011 |
| Lot number: | Batch Dependent |
| Expiration date: | Batch Dependent |
| Volume: | 50 mL |
| Content | Tris-Acetate + EDTA |
| pH | 8.3 |
| Appearance: | Clear Solution |
| Application: | Agarose DNA electrophoresis (dilute to 1X before use) |
| Shelf-Life | Two years from date of manufacture |
| Storage: | Room temperature |
| Keywords: | TAE solution, tris-acetate EDTA buffer, TAE buffer, DNA electrophoresis buffer, TAE Buffer 10X Solution |
| Grade | Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity>18 MΩ-cm) and are filtered on a 0.22 um. |
| References | 1: Strutz K, Stellwagen NC. Do DNA gel electrophoretic mobilities extrapolate to the free-solution mobility of DNA at zero gel concentration? Electrophoresis. 1998 May;19(5):635-42. doi: 10.1002/elps.1150190504. PMID: 9629889.2: Stellwagen NC, Bossi A, Gelfi C, Righetti PG. DNA and buffers: are there any noninteracting, neutral pH buffers? Anal Biochem. 2000 Dec 1;287(1):167-75. doi: 10.1006/abio.2000.4848. PMID: 11078596.3: Ungewickell E, Oestergaard L. Identification of the clathrin assembly protein AP180 in crude calf brain extracts by two-dimensional sodium dodecyl sulfate- polyacrylamide gel electrophoresis. Anal Biochem. 1989 Jun;179(2):352-6. doi: 10.1016/0003-2697(89)90143-7. PMID: 2774183.4: Hayes VM, Wu Y, Osinga J, Mulder IM, van der Vlies P, Elfferich P, Buys CH, Hofstra RM. Improvements in gel composition and electrophoretic conditions for broad-range mutation analysis by denaturing gradient gel electrophoresis. Nucleic Acids Res. 1999 Oct 15;27(20):e29. doi: 10.1093/nar/27.20.e29. PMID: 10497279; PMCID: PMC148660.5: Stellwagen NC. Apparent pore size of polyacrylamide gels: comparison of gels cast and run in Tris-acetate-EDTA and Tris-borate-EDTA buffers. Electrophoresis. 1998 Jul;19(10):1542-7. doi: 10.1002/elps.1150191004. PMID: 9719523.6: Stellwagen E, Stellwagen NC. Determining the electrophoretic mobility and translational diffusion coefficients of DNA molecules in free solution. Electrophoresis. 2002 Aug;23(16):2794-803. doi: 10.1002/1522-2683(200208)23:16<2794::AID-ELPS2794>3.0.CO;2-Y. PMID: 12210184.7: Ali RW, Martin L, Haffajee AD, Socransky SS. Detection of identical ribotypes of Porphyromonas gingivalis in patients residing in the United States, Sudan, Romania and Norway. Oral Microbiol Immunol. 1997 Apr;12(2):106-11. doi: 10.1111/j.1399-302x.1997.tb00625.x. PMID: 9227134.8: Common stock solutions, buffers, and media.Curr Protoc Cell Biol.2001 May; Appendix 2:Appendix 2A. |
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