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Molecular Depot ELISA Blocking Buffer (10X Solution) B2010010
Concentrated solution (10X) of ELISA Blocking Buffer that must be diluted with di-water to a 1X working solution before use. This buffer has been extensively validated for use in ELISA applications by coating various proteins, protein-conjugates and antibodies. This buffer can be used during the production of ELISA coated microplates.
ELISA Coating/Washing/Blocking Protocol
Detailed protocol is provided.
References
- 1: Enzyme immunoassay and enzyme-linked immunosorbent assay.Gan SD, Patel KR.J Invest Dermatol. 2013 Sep;133(9):e12.
- 2: Enzyme-linkedimmunosorbentassay (ELISA).Reen DJ.Methods Mol Biol. 1994;32:461-6.
- 3: Recent Advances in Enhancement Strategies for Electrochemical ELISA-Based Immunoassays for Cancer Biomarker Detection.Arya SK, Estrela P.Sensors (Basel). 2018 Jun 22;18(7):2010.
- 4: The application of immunoassay techniques, including enzyme-linkedimmunosorbentassay (ELISA), to snake venom research.Theakston RD.Toxicon. 1983;21(3):341-52.
- 5: Commercial enzyme-linked immunosorbent assay versuspolymerase chain reaction for the diagnosis of chronic Chagas disease: a systematic review and meta-analysis.Brasil PE, Castro R, Castro Ld.Mem Inst Oswaldo Cruz. 2016 Jan;111(1):1-19.
- 6: Lequin RM. Enzyme immunoassay (EIA)/enzyme-linked immunosorbent assay
(ELISA). Clin Chem. 2005 Dec;51(12):2415-8. - 7: Aydin S. A short history, principles, and types of ELISA, and our laboratory
experience with peptide/protein analyses using ELISA. Peptides. 2015
Oct;72:4-15. - 8: Eble JA. Titration ELISA as a Method to Determine the Dissociation Constant
of Receptor Ligand Interaction. J Vis Exp. 2018 Feb 15;(132):57334. - 9: Pang B, Zhao C, Li L, Song X, Xu K, Wang J, Liu Y, Fu K, Bao H, Song D, Meng
X, Qu X, Zhang Z, Li J. Development of a low-cost paper-based ELISA method for
rapid Escherichia coli O157:H7 detection. Anal Biochem. 2018 Feb 1;542:58-62. - 10: Major M, Law M. Detection of Antibodies to HCV E1E2 by Lectin-Capture ELISA.
Methods Mol Biol. 2019;1911:421-432. doi: 10.1007/978-1-4939-8976-8_28.
Specifications
| Catalog number: | B2010010 |
| Lot number: | Batch Dependent |
| Expiration date: | Batch Dependent |
| Volume: | 100 mL |
| Appearance: | Clear to slightly white Solution |
| Application: | ELISA |
| Shelf-Life | 3 years |
| Storage: | Room temperature |
| Keywords: | Elisa blocking protocol, ELISA blocking buffer, blocking Elisa plates, blocking buffer Elisa. |
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- Reason for return
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