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ORCA-Flash4.0 LT3 Digital CMOS camera

Thumbnail Filmstrip of ORCA-Flash4.0 LT3 Digital CMOS camera Images

    ORCA-Flash4.0 LT3 Digital CMOS camera

    SKU: C11440-42U40

    Vendor: Hamamatsu
    Part Number: hamamatsu-orca-flash4-lt3

    Product Documents

    ORCA-Flash4.0 LT3 Digital CMOS camera C11440-42U40

    The ORCA-Flash4.0 LT3 is a new scientific CMOS camera for fluorescence imaging, which has been improved from the ORCA-Flash 4.0 released in 2011.

    It is equipped with a high level of performance required for fluorescence imaging such as life science applications, and can be used not only for basic research applications but also for integration into various types of equipment.


    • Low readout noise (Standard scan): 1.5 electrons rms, 0.9 electrons median
    • High speed readout (Rapid rolling, Full resolution): 40 frames/s
    • Large field of view: 13.312 mm (H)×13.312 mm (V)
    • High resolution: 2048 (H)×2048 (V)
    • High quantum efficiency: 82 % (Peak QE)


    • Time-lapse imaging
    • Multidimensional imaging
    • Ca2+ imaging
    • DNA chip reading

    High S/N equivalent or better than BSI camera

    The graph below compares the readout noise distribution with the Gen II BSI camera* and shows that the ORCA-Flash4.0 LT3 has more pixels with smaller readout noise.

    With a low readout noise of 1.5 electrons rms, the ORCA-Flash4.0 LT3 delivers high S/N comparable to or better than a BSI camera, especially in the low light range.

    This performance can be demonstrated in high quality multidimensional imaging, as typified by long time-lapse imaging with low excitation light with low phototoxicity and photobleaching.

    * BSI camera: Back-side illuminated camera

     ORCA-Flash4.0 LT3 Readout Noise

    High speed readout

    The ORCA-Flash4.0 LT3 is capable of 40 frames/second readout, which is even faster than the conventional ORCA-Flash4.0 LT+ (30 frames/second). This is useful for applications which requires real time measurement with high temporal resolution.

    Large field of view

    The wide field of view of 13.312 mm (H) × 13.312 mm (V) allows more information to be obtained in a single shot. 

    This contributes to higher throughput in experiments such as DNA chip reading.

     ORCA-Flash4.0 LT3 Large field of view


    Product number C11440-42U40
    Imaging device Scientific CMOS image sensor
    Effective number of pixels 2048 (H) × 2048 (V)
    Pixel size 6.5 μm (H) × 6.5 μm (V)
    Effective area 13.312 mm (H) × 13.312 mm (V)
    Full well capacity (typ.) 30 000 electrons
    Dynamic range (typ.) 33 000: 1 *1 20 000: 1 *2
    Cooling method Forced-air cooled, peltier cooling
    Cooling temperature

    Forced-air cooled

    (FAN speed: Fast ambient temperature: +10 °C to +30 °C)

    +10 °C
    Frame rate at full resolution Rapid rolling 40 frames/s
    Standard scan 30 frames/s
    Readout noise (typ.) Rapid rolling 1.9 electrons rms / 1.3 electrons median
    Standard scan 1.5 electrons rms / 0.9 electrons median
    Dark current (typ.) Cooling temperature: +10 °C 0.6 electrons/pixel/s
    Digital output 16 bit
    Exposure time Internal trigger mode *3 Rapid rolling 1 ms to 10 s
    Standard scan 3 ms to 10 s

    Internal trigger mode

    with sub-array readout

    Rapid rolling 40 μs to 10 s
    Standard scan 129.99 μs to 10 s
    External trigger input mode

    Edge trigger, Global reset edge trigger,  Level trigger, Global reset level trigger,  Synchronous readout trigger, Start trigger

    Trigger delay function 0 s to 10 s in 1 μs steps
    Trigger input connector SMA
    Trigger output

    3 programmable timing outputs,  Trigger ready output, Global exposure timing output, Low output, High output

    Trigger output connector SMA
    Binning 2×2, 4×4
    Sub-array Yes
    Interface USB 3.1 Gen 1
    Lens mount C-mount
    Power supply AC 100 V to AC 240 V, 50 Hz / 60 Hz
    Power consumption Approx. 75 VA
    Ambient operating temperature FAN speed: Fast 0 °C to +40 °C
    Ambient operating humidity 30 % to 80 % (With no condensation)
    Ambient storage temperature −10 °C to +50 °C
    Ambient storage humidity 90 % Max. (With no condensation)

    *1  Calculated from the ratio of the full well capacity and the readout noise (median) in standard scan

    *2  Calculated from the ratio of the full well capacity and the readout noise (rms) in standard scan

    *3  Minimum exposure time in internal trigger mode varies depending on sub-array size and position.

    Spectral response

    ORCA-Flash4.0 LT3 spectral response


     ORCA-Flash4.0 LT3 Dimensions


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