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ELK Biotechnology Human SARS-CoV-2 Nucleocapsid ELISA Kit Size 48T ECV001
Specifications
| Product name: | Human SARS-CoV-2 Nucleocapsid ELISA Kit |
| Reactivity: | Human |
| Alternative Names: | COVID-19;SARS-CoV-2; 2019-nCoV |
| Assay Type: | Sandwich |
| Sensitivity: | 3.2 pg/mL |
| Standard: | 500 pg/mL |
| Detection Range: | 7.82-500 pg/mL |
| Sample Type: | serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Assay Length: | 3.5h |
| Research Area: | Infectious Diseases |
| Test principle: | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SARS-CoV-2 Nucleocapsid. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SARS-CoV-2 Nucleocapsid. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SARS-CoV-2 Nucleocapsid, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SARS-CoV-2 Nucleocapsid in the samples is then determined by comparing the OD of the samples to the standard curve. |
Standard curve
| Concentration (pg/mL) | OD | Corrected OD |
|---|---|---|
| 500.00 | 2.248 | 2.103 |
| 250.00 | 1.668 | 1.523 |
| 125.00 | 1.078 | 0.933 |
| 62.50 | 0.665 | 0.520 |
| 31.25 | 0.429 | 0.284 |
| 15.63 | 0.323 | 0.178 |
| 7.82 | 0.236 | 0.091 |
| 0.00 | 0.145 | 0.000 |
Precision
Intra-assay Precision (Precision within an assay)：CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)：CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery
Matrices listed below were spiked with certain level of recombinant SARS-CoV-2 Nucleocapsid and the recovery rates were calculated by comparing the measured value to the expected amount of SARS-CoV-2 Nucleocapsid in samples.
| Matrix | Recovery range | Average |
|---|---|---|
| serum(n=5) | 90-105% | 97% |
| EDTA plasma(n=5) | 79-97% | 88% |
| Heparin plasma(n=5) | 80-92% | 86% |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of SARS-CoV-2 Nucleocapsid and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Matrix | 1:2 | 1:4 | 1:8 | 1:16 |
|---|---|---|---|---|
| serum(n=5) | 95-105% | 88-102% | 95-102% | 87-98% |
| EDTA plasma(n=5) | 79-96% | 85-99% | 98-105% | 92-101% |
| Heparin plasma(n=5) | 85-105% | 92-104% | 95-102% | 86-97% |
All returns must be authorized by Spectra Services in order to insure proper credit and must be requested within 30 days of purchase. All returns are subject to a minimum 15% restocking charge. For returns not due to error on behalf of Spectra Services, the customer is responsible for all transportation fees related to the returned product. To ensure proper credit, each product return must include the following information:
- Customer name and address
- Purchase order number
- Spectra Services shipping order number
- Date of invoice
- Item number of returned item(s)
- Spectra Services return authorization number (RMA)
- Reason for return
Non-Returnable Goods
Unfortunately, Spectra Services is unable to accept the following returns at this time:
- Products not in completely resalable condition (including products with damaged, missing, or defaced labeling or packaging
- Chemicals, reagents, diagnostics, sterile, or any controlled products (unless products do not meet specification
- Laboratory apparatus or instruments that have been used or are without the original packaging, labeling and operating manuals
- Refrigerated products or other perishables
- Products which are not inventoried by Spectra Services and are not able to be returned to the manufacturer
- Products purchased on a special order
- Products not purchased from Spectra Services
- Products with an expired shelf life or an expiration date too short for resale
- Discontinued products
Spectra Services reserves the right to review this policy on a case-by-case basis.
Pre-Owned Products
Upon receipt of the pre-owned product(s), the customer has one week to evaluate the product to ensure it meets their expectations. If for some reason the customer decides to return the product, they may do so at any point during those seven (7) days for a full refund. No restocking fee will be assessed, however, the customer is responsible for the return shipping costs. Full credit will be issued upon receipt and inspection of the item(s) to ensure equipment was returned in the same condition it was initially shipped out in.
Important Information
All exchanges and returns require the completion of a Return Merchandise Authorization (RMA) form. Once completed, please return the merchandise immediately as the RMA number is only active for ten (10) days from the date of issuance.
Charges associated with returning items are the responsibility of the customer unless the item(s) arrived defective, damaged, or incorrect.
Spectra Services highly recommends that return packages be insured for full value and packaged securely to prevent shipping damage.
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I'd like to make a return. How do I do that?
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