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Bioptechs Objective Heater Medium 150819-13

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    Bioptechs Objective Heater Medium 150819-13

    SKU: 150819-13




    Vendor: Bioptechs
    Shipping Weight: 5.00 pounds
    Part Number: bioptechs-150819-13
    Our Price:   $841.23
     
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    Bioptechs Objective Heater Medium 150819-13

    The Bioptechs Objective Heater is a system specifically designed to safely and accurately warm the objective of a microscope to prevent heat loss from the specimen when using immersion lenses. There are two main components, are the electronic controller and the objective heating attachment. The Bioptechs Objective Heater System is the only objective heating system that incorporates the thermal profile of the objective into the temperature control loop and regulates the objective temperature to the specimen plane where it belongs!

    Objective Heater

    The problem

    When live-cell imaging requires the use of high numeric aperture lenses, it is necessary to control the temperature of the objective as well. This problem exists because the optical coupling medium (oil, glycerin or water) acts as a thermal coupling medium and draws heat away from the specimen. The thermal mass of a fluid coupled objective is overwhelming when compared to the thermal mass of the cells. Unfortunately microscope manufacturers do not offer temperature controlled objectives; nor do they consider the need for thermal regulation in the design of their objectives. Bioptechs has developed and patented a product that solves this problem for the majority of immersion objectives.

    The solution:

    To eliminate this thermal gradient, Bioptechs offers a patented Objective Heater® System which includes a heater/sensor and an electronic controller. The heater/sensor is comprised of an adjustable thin-film heating band which surrounds 3/4 of the diameter of the upper region of the central retracting tube of the objective. A surface probe thermal sensor positioned in the gap formed between the ends of the heating band measures the temperature of the objective. This heater/sensor assembly is supported on an adjustable metal mounting to fit objectives ranging from 14 to 37mm in diameter. The heater loop requires a minimum of 3.5 to 6mm longitudinal, physical contact with the cylindrical objective surface depending on which objective heater is required. In some cases there may be a decorative collar on the objective that must be removed in order to permit adequate surface contact. Depending on the objective, it may be necessary to invert the objective heater and mount the objective upside down for optimal thermal transfer to the lens of the objective. It may also be necessary to isolate the objective from the nosepiece turret for proper objective temperature regulation. Thermal Spacers are available from Bioptechs to reduce the heat sinking effect of the nosepiece and the other objectives on it. The Controller is specifically designed to slowly heat the objective over a fifteen minute warm-up period then hold the objective at the set point value within 0.2 degrees C. The Controller operates from ambient to 55 degrees C and has special safety circuitry which utilizes a 0.9 degrees C error window to shut down the controller and sound an alarm if, for any reason, the temperature of the objective deviates after it has reached set point. A user calibration test is also built in to the controller.

    Thermographic Objective Heater
    Thermographic image of Bioptechs Objective Heater

    Notice the temperature distribution in the following locations: nosepiece, bottom of objective, region above heater band, and top of objective. Power consumption after equilibration is 1.3 watts. The point is that, unless you warm the entire microscope, the microscope will always act as a heat sink with respect to warming the objective. You can expect a small thermal gradient.

    All objectives have different thermal profiles. Therefore, it is imperative to efficiently transfer heat to the core of the objective and prevent excess heat from radiating from the heater-band. This is exactly what the Bioptechs Objective Heater does!

    What size do I buy?

    I know my objective size:

    • Large Heater: 30-37mm
    • Medium Heater: 23-33mm
    • Small Heater: 14-22mm
    • Standard Heater: 19-27mm

    Not sure of my objective size

    If you have an objective that you are not sure how to heat you have a few options:

    1. Send the model number or writing on the objective to a Bioptechs representative.
    2. Measure your objective and select the appropriate heater.
    3. Send your objective into Bioptechs to have it sized, photographed and thermally profiled. 

    If your objective has a decorative collar which prevents an efficient heat transfer, it will be suggested to be removed. You can do this yourself with the aide of a special objective wrench and plier set available from Bioptechs or send in your objective and Bioptechs will remove it at no charge.

    Special note:

    Although there have been no formal studies relative to the effects of thermal cycling of objectives between ambient and 37 degrees C, Bioptechs has found that after selling and supporting thousands of objective warmers used for DIC, Confocal, MP, STED, STORM, SPD, Super Resolution etc. imaging, there have been no reported instances of objective damage due to daily thermal cycling between room temperature and 37 degree C. However, if you want to store the warmed objectives in a constant temperature enclosure when not in use, the Boekel desktop incubator is well suited for this application as well as a convenient place for temporary storage of specimens or media near your microscope.
    When using the Bioptechs Objective Heater, Cargille type 37 Immersion oil is highly recommended to optimize the objective’s PSF. This oil is formulated to have a refractive index of 1.515 at 37 degree C. This is an ideal optical coupling medium for in-vitro, live-cell imaging. (not for in-vivo use)

    When using the Bioptechs Objective Heater, Cargille type 37 Immersion oil is highly recommended to optimize the objective’s PSF. This oil is formulated to have a refractive index of 1.515 at 37 degree C (PN# 16237) (Cargille Webpage). This is an ideal optical coupling medium for in-vitro, live-cell imaging. (not for in-vivo use)

    Optional Accessories for the Objective Heater:

    Item: Reason:
    Wrench and Pliers Used to remove a decorative collar from an objective
    Thermal Isolators Used when cooling an objective to thermally isolate the objective from the turret of the microscope and has a double AR coated window with gas ports to introduce dry air to prevent fogging of the lower element
    Thermal Spacers Used for difficult to heat objectives to thermally isolate the objective from the turret of the microscope
    9mm Stage Spacer Kit Used to raise the stage of the microscope up 9mm to accommodate for a thermal spacer
    Cooling Collar Used to cool the objective

    All returns must be authorized by Spectra Services in order to insure proper credit and must be requested within 30 days of purchase. All returns are subject to a minimum 15% restocking charge. For returns not due to error on behalf of Spectra Services, the customer is responsible for all transportation fees related to the returned product. To ensure proper credit, each product return must include the following information:

    • Customer name and address
    • Purchase order number
    • Spectra Services shipping order number
    • Date of invoice
    • Item number of returned item(s)
    • Spectra Services return authorization number (RMA)
    • Reason for return

    Non-Returnable Goods
    Unfortunately, Spectra Services is unable to accept the following returns at this time:

    • Products not in completely resalable condition (including products with damaged, missing, or defaced labeling or packaging
    • Chemicals, reagents, diagnostics, sterile, or any controlled products (unless products do not meet specification
    • Laboratory apparatus or instruments that have been used or are without the original packaging, labeling and operating manuals
    • Refrigerated products or other perishables
    • Products which are not inventoried by Spectra Services and are not able to be returned to the manufacturer
    • Products purchased on a special order
    • Products not purchased from Spectra Services
    • Products with an expired shelf life or an expiration date too short for resale
    • Discontinued products

    Spectra Services reserves the right to review this policy on a case-by-case basis.

    Pre-Owned Products

    Upon receipt of the pre-owned product(s), the customer has one week to evaluate the product to ensure it meets their expectations. If for some reason the customer decides to return the product, they may do so at any point during those seven (7) days for a full refund. No restocking fee will be assessed, however, the customer is responsible for the return shipping costs. Full credit will be issued upon receipt and inspection of the item(s) to ensure equipment was returned in the same condition it was initially shipped out in.

    Important Information

    All exchanges and returns require the completion of a Return Merchandise Authorization (RMA) form. Once completed, please return the merchandise immediately as the RMA number is only active for ten (10) days from the date of issuance.

    Charges associated with returning items are the responsibility of the customer unless the item(s) arrived defective, damaged, or incorrect.

    Spectra Services highly recommends that return packages be insured for full value and packaged securely to prevent shipping damage.

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    249 David Parkway
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    Phone: 800-955-7732
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