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Thumbnail Filmstrip of Molecular Depot RNA Sample Loading Buffer (Molecular Biology Grade) B2010118 Images
Molecular Depot RNA Sample Loading Buffer (Molecular Biology Grade) B2010118
RNA Sample Loading Buffer is a high quality research product used as RNA loading buffer during RNA electrophoresis. Custom bulk orders of this product are available upon request.
RNA Sample Loading Buffer
Catalog number:B2010118
Lot number:Batch Dependent
Expiration Date:Batch dependent
Volume/Weight:4 x 1 mL
pH:n/a
Supplied as:Concentrate (Dilute 1:3 or 1:6 with sample before use)
Appearance:Blue Solution
Applications:RNA loading buffer during RNA electrophoresis.
Storage:−20°C
Keywords:RNA loading buffer, RNA electrophoresis buffer
Grade:Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered on a 0.22 um.
References:
1: Studier FW, Moffatt BA. Use of bacteriophage T7 RNA polymerase to direct
selective high-level expression of cloned genes. J Mol Biol. 1986 May
5;189(1):113-30.
2: Borkotoky S, Murali A. The highly efficient T7 RNA polymerase: A wonder
macromolecule in biological realm. Int J Biol Macromol. 2018 Oct 15;118(Pt
A):49-56.
3: Gholamalipour Y, Karunanayake Mudiyanselage A, Martin CT. 3′ end additions by
T7 RNA polymerase are RNA self-templated, distributive and diverse in character-
RNA-Seq analyses. Nucleic Acids Res. 2018 Oct 12;46(18):9253-9263.
4: Sarcar SN, Miller DL. A specific, promoter-independent activity of T7 RNA
polymerase suggests a general model for DNA/RNA editing in single subunit RNA
Polymerases. Sci Rep. 2018 Sep 17;8(1):13885.
5: Liang X, Li C, Wang W, Li Q. Integrating T7 RNA Polymerase and Its Cognate
Transcriptional Units for a Host-Independent and Stable Expression System in
Single Plasmid. ACS Synth Biol. 2018 May 18;7(5):1424-1435.
6: Milisavljevič N, Perlíková P, Pohl R, Hocek M. Enzymatic synthesis of base-
modified RNA by T7 RNA polymerase. A systematic study and comparison of
5-substituted pyrimidine and 7-substituted 7-deazapurine nucleoside
triphosphates as substrates. Org Biomol Chem. 2018 Aug 15;16(32):5800-5807.
7: Jin H, Lindblad P, Bhaya D. Building an Inducible T7 RNA Polymerase/T7
Promoter Circuit in Synechocystis sp. PCC6803. ACS Synth Biol. 2019 Apr
19;8(4):655-660.
8: Ji S, Thomforde J, Rogers C, Fu I, Broyde S, Tretyakova NY. Transcriptional
Bypass of DNA-Protein and DNA-Peptide Conjugates by T7 RNA Polymerase. ACS Chem
Biol. 2019 Dec 20;14(12):2564-2575.
9: Juhas M, Ajioka JW. T7 RNA polymerase-driven inducible cell lysis for DNA
transfer from Escherichia coli to Bacillus subtilis. Microb Biotechnol. 2017
Nov;10(6):1797-1808.
10: Hassan MI, McSorley FR, Hotta K, Boddy CN. Inducible T7 RNA Polymerase-
mediated Multigene Expression System, pMGX. J Vis Exp.2017 Jun 27;(124):55187.
protein synthesis reveals insights into the regulation of T7 RNA p
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