The pE-2 is a fluorescence LED illumination system which provides repeatable and controllable light with a choice of 20 different LED peaks. It is perfect for the researcher who has a specific set of tests to conduct. The user can specify a wavelength configuration matched to their particular fluorescence requirements. It is modular, allowing LED wavelengths to be exchanged as requirements vary or additional experiments are added. Manual control is via a remote control pod with instant on/off and intensity control from 0-100%. Additionally, the system is fully integrated under many imaging software packages.The mercury-free pE-2 LED illumination system is designed for use on most epi-fluorescent microscopes. The output is stable, repeatable and controllable. High intensity light is generated within the pE-2 Light Source and delivered to the microscope via two liquid light guides and a combining collimator.
This modular system accepts LAMs (LED Array Modules) selected from CoolLED’s wide range of LED wavelengths. These LAMs can be interchanged by the user so that different LED wavelengths can be illuminated depending on the application. The system can drive up to 4 LED wavelengths.
Recent developments in LED technology have enabled increased intensity in what was historically the weaker green-yellow-red (“GYR”) region of the LED spectrum. A new wide spectrum GYR LAM is now available and can readily be fitted to new and existing pE-2 systems. The graph below shows the greater comparative intensity of this LAM over the previous LED wavelengths available for this spectral region.
The system comprises a pE-2 Light Source which powers and thermally controls the LAMs, two liquid light guides, a combining collimator fitted with a microscope adaptor, and a control pod. The collimator includes an exchangeable dichroic mirror which is matched to combine the selected LED wavelengths from each installed LAM.
Excitation light can be filtered within the pE-2 system instead of within the microscope so that the need for an excitation filter wheel for dual staining and other fast-switching applications is removed. For this purpose, two filter holders are provided with the collimator. When populated with excitation filters, these can be inserted into the optical path within the collimator.
With an expected lifetime in excess of 25,000 on hours and a comprehensive range of LED wavelengths and microscope adaptors, the pE-2 can be fitted to most current and older microscopes and operate for many years without aligning or replacing bulbs. The result is a versatile and configurable fluorescence illumination system without any additional operating costs.