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Semrock LED-DA-FI-TR-Cy5-4X4M-A-000 BrightLine Sedat Filter Set
    Semrock LED-DA-FI-TR-Cy5-4X4M-A-000 BrightLine Sedat Filter Set
    Purchase Semrock LED-DA-FI-TR-Cy5-4X4M-A-000 BrightLine Sedat Filter Set
    • Semrock LED-DA-FI-TR-Cy5-4X4M-A-000 BrightLine Sedat Filter Set

      SKU: LED-DA-FI-TR-Cy5-4X4M-A-000
      Product Code: LED-DA-FI-TR-Cy5-4X4M-A-000
      Vendor: Semrock
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    BrightLine® Sedat filter set, optimized for DAPI, FITC, TRITC, & Cy5 and other like fluorophores, illuminated with LED-based light engines

    Semrock’s LED-based light engine filter sets are designed to use common excitation filters between single-band and corresponding multiband configurations to simplify interchangeability when installing the excitation filters into the light engine. This not only provides seamless transition between using single-band and multiband filter sets but also provides significant cost savings. 

    This 9-filter "Sedat" quad-band set is designed for high-speed, sequential imaging of DAPI, FITC, TRITC, and Cy5 (and other key fluorophores – see complete list above). This set has been designed to deliver significant fluorescence signal improvements for LED-based light engines compared with traditional sets designed for standard broadband light sources such as mercury or xenon arc lamps. The complete set is comprised of a quad-band beamsplitter with four single-band emitters intended to be mounted in filter wheels and four single-band exciters which can be installed into the LED light engine or mounted in filter wheels.

     

    Optical Specifications
    Specification Value
    Blocking BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

    General Filter Specifications
    Specification Value
    Microscope Compatibility BrightLine filters are available to fit Leica, Nikon, Olympus, and Zeiss microscopes.
    Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

    Physical Filter Specifications
    Specification Value
    Scratch-Dig 60-40
    Exciter/Emitter Orientation Arrow on ring indicates preferred direction of propagation of light.
    Dichroic Orientation "Reflective coating side" should face toward light source and sample.