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Semrock FRET-CFP-YFP-C-000 BrightLine FRET Filter Set

Semrock FRET-CFP-YFP-C-000 BrightLine FRET Filter Set
    Semrock FRET-CFP-YFP-C-000 BrightLine FRET Filter Set
    Purchase Semrock FRET-CFP-YFP-C-000 BrightLine FRET Filter Set

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  • BrightLine® FRET filter set, optimized for Cyan & Yellow Fluorescent Protein and other like fluorophores

    This four-filter set is specially designed to achieve high-performance fast FRET (Fluorescence Resonance Energy Transfer) imaging for cyan donor fluorescence and yellow acceptor fluorescence. FRET measurements using wide-field microscopy are challenging – using the best possible filters can make a significant difference. These filter combinations provide excellent brightness and signal-to-noise ratio while minimizing the undesirable donor-acceptor crosstalk.


    Optical Specifications
    Specification Value
    Blocking BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker™ fluorescence filter design.

    General Filter Specifications
    Specification Value
    Microscope Compatibility BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
    Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6.0 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/cm2) for over 500 hours.

    Physical Filter Specifications
    Specification Value
    Scratch-Dig 60-40
    Exciter/Emitter Orientation Arrow on ring indicates preferred direction of propagation of light.
    Dichroic Orientation "Reflective coating side" should face toward light source and sample.



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