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Bioptech FCS2 Environmental Chambers
    Bioptech FCS2 Environmental Chambers
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    • Bioptech FCS2 Environmental Chambers

      Product Code: FCS2
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    Biotech FCS2
    Bioptech FCS2
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    The Focht Chamber System 2 (FCS2) is a closed system, live-cell micro-observation chamber, that offers several advantages over other chambers. In addition to its unique perfusion and thermal control systems it is fully compatible with all modes of microscopy. It is also the only chamber to combine high-volume laminar flow perfusion rates with Koehler illumination and precise temperature control without an air curtain.

    The system is comprised of:

    • Chamber (environmental optical cavity, this page)
    • Electronic Controller (this page)
    • Stage Adapter
    • Objective Heater
    • Objective Temperature Controller

    Technical Specifications

    Physical Size 75mm OD 13mm high
    Coverslip No. 1.5 thick x 40mm Diameter
    Imaging Aperture 22mm
    Maximum Volume 706mm^3
    Minimum Volume <31mm^2
    Maximum Volume Exchange Rate 1/sec
    Minimum Fluid Aperture 0.6mm^2
    Separation between optical surfaces 50 - 1000 microns
    External port ID 1.6mm
    Temperature Stability +/- 0.2 degrees C

    *Specifications subject to change without notice.

    How it works:
    Cells are plated on a 40mm coverslip and placed into a special FCS2 chamber. This provides a perfuseable laminar flow optical chamber with user modifiable flow characteristics. The upper glass element (Microaqueduct slide) is then used to remove heat from the specimen cavity to be absorbed in the cooled fluid being circulated in the cavity formed by the addition of an O-ring sealed window adapter. Gravity or an electric pump is then used to create a flow of chilled fluid through the heat exchange cavity in the chamber. The cells remain safely enclosed in a separate optical enclosure. If more thermal transfer is necessary, tubing can be installed into the FCS2 base.

    Note: If you are using high N.A. objectives at below ambient temperatures it will be necessary to cool and thermally isolate the objective from the scope. The optional thermal isolator will prevent condensation from forming on the lower element of the objective. A gas port is provided to purge the adapter with dry nitrogen. An anti-reflection coated window optimized for 340-700nm seals the bottom of the adapter.

    Objective Cooling Collar
    The Objective Cooling Collar is an isolated fluid and thermal transfer device that attaches to the top of the objective and provides a means of attaching coolant lines from a cold source or chiller bath.  The removal of energy from the objective is more difficult and less efficient than heating.  Make sure you have enough sub stage room for the additional diameter required for the cooling collar and tubing.  Cooling collars are precision machined to fit specific objectives.  Please call Bioptechs for additional compatibility information.