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CFP/YFP/HcRed-3X-A BrightLine Pinkel filter set
    CFP/YFP/HcRed-3X-A BrightLine Pinkel filter set
    Purchase CFP/YFP/HcRed-3X-A BrightLine Pinkel filter set
    • CFP/YFP/HcRed-3X-A BrightLine Pinkel filter set

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      Product Code: CFPYFPHcRed3XA
      Vendor: Semrock
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    From Semrock  
      Semrock CFP/YFP/HcRed-3X-A BrightLine Pinkel filter set, optimized for Cyan & Yellow Fluorescent Proteins & HcRed and other like fluorophores
     
    This 5-filter "Pinkel" set is perfectly optimized for imaging CFP, YFP, and HcRed with high brightness, extremely low crosstalk, and superb signal-to-noise ratio. The set is also ideal for samples triple-labeled with CFP, YFP, and an orange or red dye, like Texas Red. It is comprised of three single-band exciters along with a triple-band beamsplitter and a triple-band emitter, and thus works best with an exciter filter wheel or slider.
    Specifications
     
    Optical Specifications:  
    Blocking BrightLine filters have blocking far exceeding OD 6 as needed to ensure a black background, even when using modern low-noise CCD cameras. The blocking is optimized for microscopy applications using our exclusive SpecMaker fluorescence filter design.
    General Filter Specifications:
    Microscope Compatibility BrightLine filters are available to fit Leica, Nikon, Olympus, Zeiss, and Aperio microscopes.
    Optical Damage Rating Testing has proven to show no signs of degradation when exposed to at least 6 W of power from an unfiltered xenon arc lamp over a 25 mm diameter (corresponding to 1.2 W/mm2) for over 500 hours.
    Phisical Filter Specifications:
    Scratch-Dig 60-40
    Exciter/Emitter Orientation Arrow on ring indicates preferred direction of propagation of light.
    Dichroic Orientation "Reflective coating side" should face toward light source and sample.
     
    Fluorophore:  
    This filter set was specifically optimized for the fluorophores highlighted in bold.
    “Compatible” indicates an excellent spectral match between fluorophore and filter set that should result in nearly ideal performance in most situations. “Acceptable” indicates a good match between fluorophore and filter set, with actual performance dependent on other experimental conditions
    Fluorophore
    Compatibility
    Aqua 431
    Acceptable
    ATTO 425
    Acceptable
    BD Horizon V450
    Acceptable
    BD Horizon V500
    Acceptable
    Cerulean
    Compatible
    CFP (ECFP)
    Compatible
    DEAC
    Compatible
    DY-415
    Compatible
    ECFP
    Compatible
    LIVE-DEAD Fixable Violet Dead Cell Stain
    Acceptable
    mBBr+GSH
    Acceptable
    Pacific Blue™
    Acceptable
    POPO-1
    Compatible
    PO-PRO-1
    Compatible
    SpectrumAqua
    Acceptable
    SYTOX Blue
    Compatible
    TagBFP
    Acceptable
    TagCFP
    Acceptable


       
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