DRIVING RESEARCH SINCE '77
Secure Checkout
PhaseView Alpha3 Light Sheet Microscope

Thumbnail Filmstrip of PhaseView Alpha3 Light Sheet Microscope Images

    PhaseView Alpha3 Light Sheet Microscope





    Vendor: PhaseView
    Part Number: phaseview-alpha3

    Product Documents

    PhaseView ALPHA3


    Alpha3 is a new generation of light sheet microscope addressing the needs of high temporal resolution along with spatial high-resolution to achieve qualitative and quantitative 3D imaging of fixed or live biological specimen.

    From in-vivo imaging to large cleared samples, the Alpha3 microscope delivers unprecedented image quality while keeping the necessary flexibility and modularity expected for cutting-edge scientific research instruments.

    Typical applications of Alpha3 include in toto imaging of small animal models such as whole mouse embryos, morphogenesis and embryogenesis of model organisms: C.elegans, Drosophila, Zebra fish, live imaging of cell cultures, functional imaging of neuronal activity, fluorescence imaging of marine organisms or plant developmental biology.


    Features


    From in-vivo imaging to large cleared samples 
    Macro to micro view imaging, from whole organs at sub-cellular resolution to very small specimen; Compatible with all clearing solutions: aqueous buffers and organic solvents

    From very small to large specimen 
    Versatile chamber with easy access; Multiple accessories provided for sample mounting to accommodate a large variety of specimens

    Smart light sheet illuminators
    Multi-directional light sheet performing real time laser focus sweeping to extend the thinnest focus area over the entire field of view, artefacts-free imaging and ultra fast Z-stacking

    Modular light sheet microscope 
    Flexible imaging system with a large selection of detection objectives: 2X to 60X,  air, dipping lenses, clearing objectives with correction collar for RI matching; Fully flexible for easy adaptation to any experiments; Open microscopy system for multimodal optical microscopy: FLIM, FRET, FRAP, photo ablation.


    Software


    Light source

    Laser combiner with up to 4 laser lines: fibered lasers CW / Laser diodes or DPSS
    Wavelength selection from 405 nm to 785nm, output power from 25 to 250mW.

    Light sheet

    Dual smart illuminators with fibered connection to laser combiner.
    Multi-directional light sheet with real time focus sweeping
    Chromatic correction 400 ‐ 632nm
    Minimum light sheet thickness 2µm, range: 2µm - 12µm, width: 2mm - 15mm

    Chamber & Holders

    Chamber dimensions : width 21mm, length 70mm height 25mm; volume < 15ml
    Chamber highly resistant to various corrosive medium, clearing agents, sea water, 
    Sample size from µm to cm range 
    Multiple holders for sample mounting: molds, coverslips , glass supports 
    Optional: temperature and CO2 controls. 

    Volume scanning

    Z motorized stage: range 15mm, precision 0.1µm, acquisition speed 40 fps
    Optional: XY motorized stages for tiling: range 15mm, precision 0.1µm
    Optional: ultra fast 3D scanning module (75 images /second)

    Detection unit

    Fluorescence microscope stand comprising objective slider 2 positions , eyepieces, video port, motorized filter wheel, multi-notch filter

    Detection lenses

    Large selection of long working distance objectives : air ,dipping lenses, clearing objectives with correction collar RI 1.33 - 1.56 
    Magnification: 2X to 60X 

    Image Sensor

    sCMOS 2048 x 2048 pixels, format 13 x 13 mm, 6.5µm square pixels., USB3 / CameraLink Interface

    Software

    QtSPIM software for stacks, XY tiling and time lapse acquisition. Easy export of raw images and metadata to open source or commercial 3rd party software

    PC

    Desktop i7-7700K 4.2Ghz – 2 x 16Go RAM - GPU GeForce GTX1060-6Go - Hard Disk 4 x 4To RAID0 - Hard Disk SSD 500Go , QHD Screen 31.5”, Windows 10 / 64b Pro

     


    Software


    From sample mounting to image acquisition, Alpha3 performs seamless light sheet imaging. The QtSPIM software provides clear and intuitive interface to collect X,Y,Z,θ,T,λ images at maximum speed. QtSPIM controls all image acquisition parameters. Along with a workstation grade computer with an optimized configuration, the microscopy system performs ultra fast acquisition Raw image data with their metadata are saved in 16 bits TIFF format, compatible with open source or commercial software for further 3D display and analysis

    All returns must be authorized by Spectra Services in order to insure proper credit and must be requested within 30 days of purchase. All returns are subject to a minimum 15% restocking charge. For returns not due to error on behalf of Spectra Services, the customer is responsible for all transportation fees related to the returned product. To ensure proper credit, each product return must include the following information:

    • Customer name and address
    • Purchase order number
    • Spectra Services shipping order number
    • Date of invoice
    • Item number of returned item(s)
    • Spectra Services return authorization number (RMA)
    • Reason for return

    Non-Returnable Goods
    Unfortunately, Spectra Services is unable to accept the following returns at this time:

    • Products not in completely resalable condition (including products with damaged, missing, or defaced labeling or packaging
    • Chemicals, reagents, diagnostics, sterile, or any controlled products (unless products do not meet specification
    • Laboratory apparatus or instruments that have been used or are without the original packaging, labeling and operating manuals
    • Refrigerated products or other perishables
    • Products which are not inventoried by Spectra Services and are not able to be returned to the manufacturer
    • Products purchased on a special order
    • Products not purchased from Spectra Services
    • Products with an expired shelf life or an expiration date too short for resale
    • Discontinued products

    Spectra Services reserves the right to review this policy on a case-by-case basis.

    Pre-Owned Products

    Upon receipt of the pre-owned product(s), the customer has one week to evaluate the product to ensure it meets their expectations. If for some reason the customer decides to return the product, they may do so at any point during those seven (7) days for a full refund. No restocking fee will be assessed, however, the customer is responsible for the return shipping costs. Full credit will be issued upon receipt and inspection of the item(s) to ensure equipment was returned in the same condition it was initially shipped out in.

    Important Information

    All exchanges and returns require the completion of a Return Merchandise Authorization (RMA) form. Once completed, please return the merchandise immediately as the RMA number is only active for ten (10) days from the date of issuance.

    Charges associated with returning items are the responsibility of the customer unless the item(s) arrived defective, damaged, or incorrect.

    Spectra Services highly recommends that return packages be insured for full value and packaged securely to prevent shipping damage.

    We're Here To Help

    Your satisfaction is important to us! Use this form Contact Us to email us your questions about products, online orders, store experiences and more.

    Order Questions:

    If you need help or have any other questions concerning your orders, please click on the "Contact us" form above or call: 800-955-7732.

    Our Address:
    Spectra Services, Inc.
    249 David Parkway
    Ontario, NY 14519 US
    Phone: 800-955-7732
    I'm having a hard time searching for a product.

    That's no problem. We're more than happy to assist you in your search. Give us a call, or drop us a line and let us know what you're looking for as best you can. We're always adding partner brands, and it's difficult to keep up with the thousands of products that get added to our lineup. Our sales specialists know our lineup best, and are most willing to help you find the solution(s) you need!

    Why can't I complete my order online?

    You most likely have one or more items in your shopping cart that are not priced. If you find that you can't complete an order, contact us via phone or email for further assistance.

    What types of payments do you accept?

    That's an easy one. We're very flexible when it comes to payment. Not only do we accept most major credit cards (Visa, Master Card, Discover, American Express), we also offer you the flexibility of paying by check, money order, wire transfers, and purchase orders. Work with any one of our sales specialists to figure out which method is best for you!

    Do you charge sales tax?

    Currently, we are authorized to collect sales tax in California, Connecticut, Florida, Indiana, Massachusetts, New Jersey, New York, Ohio, Pennsylvania, Texas and Washington. If we ship to any of these states, we are obligated to charge sales tax unless you provide us with a tax exempt certificate. You can either fax a copy of the certificate to our New York office (585.265.4374) or email it to ([email protected]).

    Do you offer special pricing for government and educational entities?

    Absolutely! Contact any one of our sales specialists to work out the details!

    I just placed an order. Can I modify/cancel it?

    We pride ourselves on providing you with a responsive and efficient buying experience. We are able to address your concerns in an accurate and timely fashion, and can usually ship your order within 24 hours of order placement. If you contact us within that window, we'll do our absolute best to accommodate your needs and concerns prior to shipment.

    Do you accept international orders?

    Of course! While we require a bank wire prior to shipment of the product on international orders, we assure you that the process is just as smooth for domestic orders. Take a look at our International Shipping Policy and contact us if we can assist.

    Will I be charged for shipping?

    Shipping charges will be added to your invoice unless you provide us with your UPS, DHL, or FedEx account number. For a more detailed insight into our shipping process, see our shipping information page.

    I received a damaged shipment. What can I do?

    Accidental damage during shipment can sometimes happen. When you take delivery of your freight, please inspect thoroughly for any visible damage to the outside of the shipping container before signing for it. If there are multiple pieces to the shipment, please account for all them. Should you find that any of the products are damaged or missing after receipt, contact us within three days to address the matter.

    I'd like to make a return. How do I do that?

    Take a look at our Return Policy, and then contact us to continue with the process. Let us know if you have any questions.

    Can you guarantee pricing and availability?

    While we attempt to list and provide you the most up-to-date pricing, we reserve the right to make changes. Additionally, we cannot guarantee that we will always have a product in stock, but we do our best to accommodate your needs. If you have questions regarding pricing or availability, please do not hesitate to ask.

    Can you explain where you come up with the 'List Price,' please?

    Our "List Price" represents the full retail price as suggested by the supplier or manufacturer, the price estimated based on comparable items offered elsewhere in the marketplace, a former price the item was offered for by Spectra Services, or the price estimated in accordance with industry standards. Please note that we do our best to provide accurate list prices, and that it may vary by geographic location.