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Semrock BrightLine Fluorescence Microscopy Filters
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The highly acclaimed BrightLine™ series of optical fluorescence filters is revolutionizing fluorescence instruments – from microscopes to microarrays. Semrock has combined the most advanced optical filter manufacturing technology in the industry with its thorough understanding of fluorescence systems and fluorescence filter design to produce the brightest and most durable fluorescence filters available anywhere. In fact, BrightLine™ filter technology is so unique that it is patent pending.

Semrock's BrightLine™ fluorescence filters are brighter, provide better contrast, and have been proven to be more durable than conventional filters (you can even clean them with alcohol or acetone!). We stock the following high-performance fluorescence filters and sets optimized for fluorescence microscopy applications.

Singleband Filters and Sets for Fluorescence Microscopy

FF409 DAPI, Hoechst, AMCA, BFP, Alexa Fluor 350
FF495 GFP, EGFP, DiO, Cy2™, YOYO-1, YO-PRO-1
FF506 FITC, rsGFP, Bodipy, FAM, Fluo-3, Alexa Fluor 488
FF555 TRITC, Rhodamine, DiI, 5-TAMRA, Alexa Fluor 532
FF562 Cy3™, PE, TAMRA, dsRed, Alexa Fluor 546 & 555
FF593 Texas Red®, Cy3.5™, 5-ROX, Alexa Fluor 568 & 594
FF660 Cy5™, APC, Alexa Fluor 647 & 660

BrightLine ZERO™ Filter Sets – Semrock has uniquely solved the problem of poor image registration—now all you have to do is choose BrightLine ZERO™ fluorescence filter sets. All BrightLine™ singleband filter sets are optionally available with certified zero-pixel-shift performance.

Multiband Filters and Sets for Fluorescence Microscopy

Semrock's multiband fluorescence filters are uniquely optimized to simultaneously provide the highest brightness, contrast, and color balance possible. We stock the following high-performance filters optimized for fluorescence microscopy applications.

DA/FI/TX Blue band: DAPI, Hoechst, AMCA, Alexa Fluor 350
Green band: FITC, GFP, rsGFP, Bodipy, Alexa Fluor 488
Red band: Texas Red®, MitoTracker Red, Alexa Fluor 568 & 594

Individual Fluorescence Filters for Instrumentation
Semrock also offers a selection of fluorescence filters that are optimized for use in a variety of fluorescence instruments. Some of these filters may be used as both excitation and emission filters, while others are better optimized for one or the other function.

Filters for OEM Instrumentation Applications
In addition to the standard catalog filters, Semrock also tailors optical filters to meet or exceed even the most demanding requirements of our volume OEM customers.

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