The original 86000 set was conceived by Dr. John Sedat, of the University of California at San Francisco, for a deconvolution imaging system developed by him and Dr. David Agard. These sets employ the use of a multi-band beamsplitter matched to single band excitation and emission filters. Most often the excitation filters are mounted in filter wheels. Be switching excitation filters and emission filters one can capture the fluorescent image of a single fluorochrome while preventing Òbleed-throughÓ of the other fluors being used. The stationery beamsplitter inhibits image shifting, as does the use of filter wheels, which cause minimal movement of the microscope.

Image by: Benjamin OÕBrien and H. Ulli Weier, University of California, E.O. Lawrence Berkeley National Laboratory, Berkeley, CA.
Filter Set: 84000