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Home > Filter Sets > Chroma 31 Series Filter Set


31-Series
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By replacing the longpass emission filter of the 11/Basic Series with bandpass emission filters Chroma's Standard series prevents bleed-through of undesired long wavelength fluorescence: for example, preventing red fluorescence from appearing in the green image). These filters also tend to decrease the problems associated with autofluorescence.

Image by: Evelin Shrûck, Stan du Manoir, Thomas Ried Filter Set: Chroma Technology Corp

Technical Specification

Set ID Set Name Set Price Part Number Type Price Set Description Notes
31000 DAPI/Hoechst/ AMCA $400 D360/40x EX $150
400DCLP BS $125
D460/50m EM $150
31001 FITC/RSGFP/ Fluo 3/ DiO Acradine Orange(+RNA) $400 D480/30x EX $150
505DCLP BS $125
D535/40m EM $150
31002 TRITC (Rhodamine)/ DiI/ Cy3ú $400 D540/25x EX $150
565DCLP BS $125
D605/55m EM $150
31002a TRITC (Rhodamine)/ DiI/ Cy3ú For redder signal $400 D540/25x EX $150 Will result in a redder image by eye as compared to the 31002 set. Will also result in a loss of total signal with Rhodamine-like dyes as compared to the 31002 set.
565DCLP BS $125
D620/60m EM $150
31003 R and B Phycoerythrin (PE) Hq Light Source $400 D546/10x EX $150 Recommended for use with mercury lamps only.
560DCLP BS $125
D580/30m EM $150
31004 Texas Red¨ / Cy3.5ú $400 D560/40x EX $150
595DCLP BS $125
D630/60m EM $150
31005 Propidium Iodide (PI) $400 D540/25x EX $150
565DCLP BS $125
D630/60m EM $150
31006 Allo-phycocyanin (APC) $400 D595/40x EX $150 The emission at these wavelengths will be better detected by a camera than by the unaided eye. Only a small percentage of humans can detect these wavelengths.
625DCLP BS $125
D660/40m EM $150
31007 Quinacrine Mustard $400 D425/40x EX $150
460DCLP BS $125
D540/50m EM $150
31008 Ethidium Bromide $400 D540/25x EX $150
565DCLP BS $125
D605/55m EM $150
31009 Cascade Blue $400 D360/40x EX $150
400DCLP BS $125
D460/50m EM $150
31010 Lucifer Yellow $400 D425/40x EX $150
460DCLP BS $125
D540/50m EM $150
31011 Acridine Orange (+RNA) $400 D480/60x EX $150 This set is designed to image Acridine Orange bound to RNA.
515DCLP BS $125
D630/60m EM $150
31012 FURA Red $400 D490/20x EX $150 Designed for non-ratio imaging of Fura Redú. For ratiometric imaging of Fura Redú, use set 71003.
515DCLP BS $125
D660/50m EM $150
31013v2 Narrow Band Excitation Set for DAPI/ Hoechst/ AMCA $400 D365/10x EX $150 Recommended for use with mercury lamps only.
400DCLP BS $100
D460/50m EM $150
31014 Wide band excitation set for Ethidium Bromide, TRITC, Phycoerythtin, DiI $400 D535/50x EX $150 The wide excitation filter can sometimes excite fluorochromes like FITC in dual-stain applications.
565DCLP BS $125
D605/55m EM $150
31015 Auramine $400 D460/50x EX $150
500DCLP BS $125
D550/50m EM $150
31016 Hydro-xycoumarin $400 D405/20x EX $150
425DCLP BS $125
D460/50m EM $150
31017 Chlorophyll Bandpass Emission $400 D480/30x EX $150
505DCLP BS $125
D660/50m EM $150
31017a Chlorophyll Longpass Emission $400 D480/30x EX $150
505DCLP BS $125
E600LP EM $150
31018 NBD / HPTS $400 D460/50x EX $150
500DCLP BS $125
D535/40m EM $150
31019 GFP $400 D425/40x EX $150 Designed for wtGFP imaging. This set has been superceded by the 41015 set.
460DCLP BS $125
D505/40m EM $150
31020 Di-8-ANEPPS (non ratio) $400 D480/30x EX $150 Designed for non-ratio imaging of Di-4-ANEPPS and Di-8-ANEPPS.
505DCLP BS $125
D605/55m EM $150
31021 Blue GFP Bandpass Emission $400 D390/22x EX $150
420DCLP BS $125
D460/50m EM $150
31023 Cy5ú $400 D640/20x EX $150 Most people cannot see Cy5ú emission by eye.
660DCLP BS $125
D680/30m EM $150
31024 DiA $400 D480/30x EX $150
505DCLP BS $125
D605/55m EM $150
31025 SPQ $400 D345/10x EX $150 This set will work better with a mercury lamp rather than a xenon lamp.
400DCLP BS $125
D445/40m EM $150
31026 Narrow-band GFP for eliminating auto-fluorescence $400 D480/20x EX $150 This set will, in some cases, increase the signal-to-noise ratio minimizing auto fluorescence of GFP imaged in live cells with high levels of auto fluorescence. It will decrease the GFP signal substantially and should not be used with dim samples.
505DCLP BS $125
D520/20m EM $150
31027 Cy5.5ú $400 D660/20x EX $150 Light passed through this emission filter cannot be seen by eye and must be detected with an IR-sensitive detector.
680DCLP BS $125
D700/30m EM $150
31028 CTC $400 D450/50x EX $150
480DCLP BS $125
D630/60m EM $150
31029 Cy5.5ú (Red Shifted) $400 D675/20x EX $150 Light passed through this emission filter cannot be seen by eye and must be detected with an IR-sensitive detector.
690DCLP BS $125
D715/30m EM $150
31030 FM 143ú $400 D480/30x EX $150
505DCLP BS $125
E570LP EM $150
31031 BFP to GFP FRET (LP) $400 D390/22x EX $150 FRET is extremely difficult in wide-field microscopy and LP emitters are not recommended except under very specific conditions. Please see our FRET application note for more information.
420DCLP BS $125
E500LP EM $150
31032 BFP to GFP FRET (BP) $400 D390/22x EX $150 FRET is extremely difficult in wide-field microscopy. Please see our FRET application note for more information.
420DCLP BS $125
D525/50m EM $150
31033 BFP to GFP FRET (LP) Wide Excitation $400 D380/30x EX $150 FRET is extremely difficult in wide-field microscopy and LP emitters are not recommended except under very specific conditions. Please see our FRET application note for more information.
420DCLP BS $125
E500LP EM $150
31034 BFP to GFP FRET (BP) Wide Excitation $400 D380/30x EX $150 FRET is extremely difficult in wide-field microscopy. Please see our FRET application note for more information.
420DCLP BS $125
D525/50m EM $150
31035 Per CP $400 D480/30x EX $150
505DCLP BS $125
D675/50m EM $150
31036v2 Aqua $400 D436/20x EX $150
455DCLP BS $125
D480/30m EM $150
31037 Pacific Blue $400 D410/40x EX $150
440DCLP BS $125
D470/40m EM $150
31038 Cascade Yellow $400 D425/40x EX $150
460DCLP BS $125
D550/40m EM $150
31039 JP1 (for EGFP when ysed with YGFP) $400 D470/20x EX $150 EGFP and EYFP are extremely difficult to separate in any imaging device. Please see Hagting, A., Jackman, M., Simpson, K. and Pines, J. (1999) 'The translocation of cyclin B1 to the nucleus at prophase requires a phosphorylation-dependent nuclear import signal'. Current Biology, 9, 680-689. for more details.
490DCLP BS $125
D510/20m EM $150
31040 JP2 (for YGFP when used with EGFP) $400 D510/20x EX $150 EGFP and EYFP are extremely difficult to separate in any imaging device. Please see Hagting, A., Jackman, M., Simpson, K. and Pines, J. (1999) 'The translocation of cyclin B1 to the nucleus at prophase requires a phosphorylation-dependent nuclear import signal'. Current Biology, 9, 680-689. for more details.
530DCLP BS $125
D560/40m EM $150
31041 Blue GFP II $400 D380/30x EX $150
420DCLP BS $125
D460/50m EM $150
31043 Sapphire/UV GFP $400 D395/40x EX $150
425DCLP BS $125
D510/40m EM $150
31044v2 Cyan GFP $400 D436/20x EX $150 This set will typically exclude the signal from YFP but not from GFP.
455DCLP BS $125
D480/40m EM $150
31046 Red $400 D577/20x EX $150
595DCLP BS $125
D630/30m EM $150
31047v2 Coumarin $400 D405/10x EX $150
425DCLP BS $100
D460/40m EM $150
31048 BFP to GFP FRET Narrow Emission $400 D390/22x EX $150 FRET is extremely difficult in wide-field microscopy. Please see our FRET application note for more information.
425DCLP BS $125
D515/30m EM $150
31050 Sapphire/ Topaz FRET $400 D395/40x EX $150 FRET is extremely difficult in wide-field microscopy. Please see our FRET application note for more information.
425DCLP BS $125
D535/30m EM $150
31051 CFP/DsRed FRET $400 D436/20x EX $150 FRET is extremely difficult in wide-field microscopy. Please see our FRET application note for more information.
455DCLP BS $125
D620/60m EM $150
31052 Cyan/ Topaz Energy Transfer $400 D436/20x EX $150 FRET is extremely difficult in wide-field microscopy. Please see our FRET application note for more information.
455DCLP BS $125
D535/30m EM $150
31053 Wide Emission CFP $400 D436/10x EX $150 Recommended for use with mercury lamps only.
450DCLP BS $125
D480/60m EM $150
31054 GFP - Wide Blue Excitation $450 D455/70x EX $150
500DCXR BS $175
D525/30m EM $150
31056 DHE $400 D335/20x EX $150
365DCLP BS $125
D405/40m EM $150
31057 Pyrene $400 D360/40x EX $150
400DCLP BS $125
D480/60m EM $150
31058 FM-464 $400 D510/40x EX $150
540DCLP BS $125
D640/50m EM $150
31059 Narrow Blue $400 D480/20x EX $150
505DCLP BS $125
D535/40m EM $150
31060 Elf-97 $400 D360/40x EX $150
400DCLP BS $125
D535/50m EM $150
31061 Xylenol Orange $600 D380/30x EX $150
420DCLP BS $125
D605/55m EM $150
31062 Fast Redú $600 D360/40x EX $150
400DCLP BS $125
E550LP EM $150

*Specifications subject to change without notice.
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