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The
most fundamental of Chroma's filter sets. These sets
were designed primarily for viewing the fluorescence
of single fluorochrome. All of these sets contain
longpass emission filters. Longpass emission filters
cannot exclude the fluorescence of a second fluorochrome
which may also be excited. For instance, blue light,
which is the primary excitation color for FITC, will
also excite, albeit slightly less efficiently, than
TRITC. A longpass emission filter will be unable to
exclude the fluorescence of TRITC. Most of the barrier
filters of our Basic sets are made from absorptive
glass.
Image by:Dr. Steve Kay and Dr. Jeff Plautz, University
of Virginia Filter Set: Chroma Technology Corp
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