Image-Pro Plus v.7.0 MediaCybernetics Image Analysis Software

Process & Enhance Features:

Use sophisticated image processing and enhancement tools to uncover details within your images.

Imaging Tiling:

Tile together a series of images or align sequence images to see a full representation of your research sample.


Before Tiling	After Tiling

Image Alignment:

Automatically align either individual or sequence images.

Image Alignment

Extended Depth of Field:
Create a focused image from a series of unfocused images with Extended Depth of Field.

Extended Depth of Field

Filter and Enhance:

Choose from a variety of enhancement and edge filters to filter noise and improve image detail.

Work with Filter dialogs that include descriptions and interactive preview windows
Use Enhancement filters that include Low-Pass, Hi-Pass, Gaussian, Hi-Gaussian, Sharpen, Flatten, Median,
Rank, Local Equalization, and De-speckle in user-definable kernel sizes
Outline objects with various Edge Filter methods including Sobel, Roberts, Laplace, Variance, Phase, Horizontal, and Vertical
Process objects with Morphological filters including Erode, Dilate, Open, Close, Top Hat, Well, Branch/End,
Watershed, Thinning, Pruning, Distance, and Reduce in user-definable kernel sizes
Work with Large Spectral Filters: Fast-acting, large kernel size (up to 4000 x 4000) filters that include Low-Pass, Hi-Pass, Band-Pass, and Edge filters
Apply filters to individual frames or to entire sequence
Customize kernel files for morphological and convolution filters


Original image	Sobel edge filter applied

Features:

Click on the icons below to learn about Image-Pro's powerful features
Acquire	Process & Enhance	Measure & Classify

3D Analysis	Surface Inspection	Automate & Share

System Requirements:

Operating System	Computer	RAM	Storage
Windows® XP Pro	750 MHz or better	1 GB RAM*	20 GB**
Windows® Vista Business and Ultimate (32 and 64 bit)***	2.1 GHz	2 GB RAM*	40 GB**

*2GB RAM is recommended on all operating systems. 4GB is best on Vista operating systems.

**Suggested storage size for large image files generated by advanced cameras.

*** Image-Pro Plus is a 32-bit application. It will run on 64-bit machines.

Image-Pro Plus 7.0 File Formats

Read Support: TIFF, IPW, JPEG, JPEG 2000, EXIF JPEG, Flat (binary), GEL, TGA, BMP, PhotoCD, HDF, QED, FITS, AVI, RAW and SEQ files.
Stack & Confocal Read Support: SEQ (Image-Pro and StreamPix), STK (MetaMorph), PIC (Bio-Rad Confocal), LSM & ZVI (Zeiss), DEB and AVZ (AutoQuant Stack), LEI and LIF (Leica), DM3 (Gatan), DV (DeltaVision), and Fluoview 1000 (Olympus) files.
Write Support: TIFF, IPW, IPS, JPEG, JPEG 2000, Flat, TGA, BMP, FITS, EPS, AVI and SEQ files.
Support for 24-, 36-, and 48-bit color; 8-, 12-, and 16-bit grayscale as well as 32-bit floating point images.

Morphology:

Use a wide range of morphological filters to precisely segment structures and prepare images for automatic measurement.

Background Subtraction & Correction:
Eliminate background noise and correct uneven backgrounds in your images for improved analysis.


Original image	Background subtracted	New image

Merge Color Channels:
Use the Color Composite feature to merge multiple fluorescent images acquired as monochrome single wavelengths into a color composite image.

Co-localization:
Detect co-localization in biological specimens and graphically display the association between the two sets of data in a scatter plot.


Detect co-localization in fluorescent images. Mouse fibroblast cells - microtubules stained with FITC, mitochondria stained with Texas Red, nuclei stained with DAPI.

Pseudo-color:

Use pseudo-color to highlight features of interest in a gray scale image. Use colors to visually amplify specific intensities which are normally difficult to distinguish from their surroundings.

Fast Fourier Transforms:

Perform forward and inverse Fast Fourier Transforms (FFTs) to eliminate artifacts.

Edit the spectrum directly including Low-Pass, High-Pass, un-sharpen, spike cut, and spike boost
Save and reload data


Before FFT	After FFT