Measure & Classify Features:

Count and Classify Objects:

Count and Characterize objects using over 50 manual and automatic measurement tools. Tag objects of interest and easily sort by size or other measurement parameters.

Measure lengths, roundness, aspects, angles, areas, perimeters, and holes
Set ranges for measurements to filter unwanted objects
Group objects in classes based on measurements
Resolve clustered objects with Watershed, Auto-Split, and Cluster analysis tools
Use colors to separate and measure objects in color images
Export measurements to statistical and spreadsheet package via DDE
Display measurements as histograms & scattergrams

Threshold and measure the precent area of objects

Count and classify objects. Image courtesy of
Dr. Gabriel Corkidi Blanco, Centro de Investigacion
Aplicada y Desarollo Technologico, UNAM, Morelo, Mexico.

Threshold & Segmentation Tools:

Use threshold and segmentation tools to extract objects of interest and distinguish them from other objects or from the background image.


Original image	Threshold values are set

Image after setting threshold	Mask image based on threshold values

Calibration Tools:

Use the Image-Pro Plus calibration tools to redefine the scale of an image to express values in other units of measure. Learn more about Image-Pro Plus calibrations in the Spatial Calibration Product Note:

Calibration wizard helps you to calibrate an active image and create reference and system calibrations
Use X and Y scale bars as a nondestructive overlay
Work with intensity or spatial measurements
Create and display spatial calibration markers
Create and display system calibrations
Use pre-defined spatial calibration units
Save and recall all calibrations
Calculate derived calibrations automatically
Store information about your microscope in lens information files

Metrology Tools:

Measure features such as best-fit line, arc, and circle

Best-fit circle measurements

Measurements:

Measure the lengths, areas, perimeters, and angles of objects in your image.

Angle Measurements

System Requirements:

Operating System	Computer	RAM	Storage
Windows® XP Pro	750 MHz or better	1 GB RAM*	20 GB**
Windows® Vista Business and Ultimate (32 and 64 bit)***	2.1 GHz	2 GB RAM*	40 GB**

*2GB RAM is recommended on all operating systems. 4GB is best on Vista operating systems.

**Suggested storage size for large image files generated by advanced cameras.

*** Image-Pro Plus is a 32-bit application. It will run on 64-bit machines.

Image-Pro Plus 7.0 File Formats

Read Support: TIFF, IPW, JPEG, JPEG 2000, EXIF JPEG, Flat (binary), GEL, TGA, BMP, PhotoCD, HDF, QED, FITS, AVI, RAW and SEQ files.
Stack & Confocal Read Support: SEQ (Image-Pro and StreamPix), STK (MetaMorph), PIC (Bio-Rad Confocal), LSM & ZVI (Zeiss), DEB and AVZ (AutoQuant Stack), LEI and LIF (Leica), DM3 (Gatan), DV (DeltaVision), and Fluoview 1000 (Olympus) files.
Write Support: TIFF, IPW, IPS, JPEG, JPEG 2000, Flat, TGA, BMP, FITS, EPS, AVI and SEQ files.
Support for 24-, 36-, and 48-bit color; 8-, 12-, and 16-bit grayscale as well as 32-bit floating point images.

Calculate Thickness:

Determine the thickness of objects by calculating the mean, minimum and maximum thickness between lines.

Trace Objects:

Use the trace tool to highlight dark features on a primarily light background, such as fingerprint ridges. These features can be drawn manually or automatically. The automatic tracing tool works by tracing the skeleton of dark features.


	Automatic tracing tool

Detect Edges:

Use the Caliper Tool to detect edges and calculate repeated measurements.


	Caliper tool is used to count gear teeth.

Colocalization of Probes:

The colocalization tools in Image-Pro Plus provide a visual representation as to the location and degree of overlap between two wavelengths.


	Original fluorescence image.	Area of interest (AOI) within the scatterplot selects the colocalized areas (orange & yellow).


	Mask shows overlapping regions of red and green.

	Composite image showing FITC-labeled fibroblasts (green), Texas Red-labeled mitochondria (red).

Track Moving Objects:

Manually or automatically follow cells or other organisms as they move through time and space. Use correlation tracking to follow objects when image segmentation is difficult or not possible.


	Track and graph the changing of intensity parameters over time within an area of interest.

Features:

Click on the icons below to learn about Image-Pro's powerful features
Acquire	Process & Enhance	Measure & Classify

3D Analysis	Surface Inspection	Automate & Share