|
The
original 86000 set was conceived by Dr. John Sedat,
of the University of California at San Francisco,
for a deconvolution imaging system developed by him
and Dr. David Agard. These sets employ the use of
a multi-band beamsplitter matched to single band excitation
and emission filters. Most often the excitation filters
are mounted in filter wheels. Be switching excitation
filters and emission filters one can capture the fluorescent
image of a single fluorochrome while preventing Òbleed-throughÓ
of the other fluors being used. The stationery beamsplitter
inhibits image shifting, as does the use of filter
wheels, which cause minimal movement of the microscope.
Image by: Benjamin OÕBrien and H. Ulli Weier,
University of California, E.O. Lawrence Berkeley National
Laboratory, Berkeley, CA.
Filter Set: 84000 |
